慢性胃炎胃粘膜肠化生和不典型增生超微结构及其分子生物学研究  

A comparative study on ultrastructure of chronic gastritis gastric mucosa IM,ATP and their molecular biology

作　　者：尹光耀[1] 张武宁[2] 沈小静[1] 陈一[2] 何雪芬[1] 

机构地区：[1]无锡市第三人民医院,214041 [2]复旦大学国家微分析中心

出　　处：《江苏医药》2002年第1期4-7,共4页Jiangsu Medical Journal

摘　　要：目的　探索慢性胃炎胃粘膜肠化生和不典型增生超微结构及其分子生物学的病理生理学变化。方法　对 16 8例慢性浅表性胃炎、慢性萎缩性胃炎和胃癌患者胃粘膜 ,采用组织化学染色、扫描电镜附能谱仪、透射电镜附能谱仪、图像分析仪、放射免疫法和化学发光法等现代科学技术手段 ,同步检测胃粘膜和胃粘膜上皮细胞超微结构、微量元素、DNA、cAMP和SOD ,以及3 H TdRLCT、LPO。结果　胃粘膜IM分度、分型和ATP分度 ;上皮细胞超微结构 ;胃粘膜、细胞核和线粒体的Zn、Cu、DNA、cAMP和SOD ;血清LPO和3 H TdRLCT ,在HC组、ATP 组、IM +ATP 组、IM +ATP 组、IMⅡb组和CA组的组间 ,有显著性差异 ,P <0 0 5～ 0 0 1;IM +ATP 组、IMⅡb组和CA组组间 ,无显著性差异 ,P >0 0 5。结论　ATP 、IMⅡb和CA有相似的上皮细胞超微结构与分子生物学变化 ,因而有相似的发生、发展和转归的规律 。Objective To explore ultrastructure of chronic gastritis (CG)gastric mucosa IM,ATP and their physiopathologic changes of molecular biology.Methods By means of histochemical staining,SEM with EDAX,TCM with EDAX,Image Analysis Technique,RIA and chemiluminescence method,gastric mucosa of 168 patients with chronic superficial gastritis (CSG),chronic atrophic gastritis (CAG) and gastric cancer (GC) were synchronously detected in terms of gastric mucosa and its epithelial cell ultrastructure,trace elements,DNA,cAMP,SOD, 3H-TdR LCT and LPO.Results In gastric mucosa IM grading,classification and ATP grading,epithelial ultrastructure,Zn,Cu,DNA,cAMP and SOD,of gastric mucosa,nuclei and mitochondria,LPO and 3H-TdR LCT,there were significant differences between healthy control group,ATP  group,IM +ATP  group,IM +ATP  group,IMⅡb group and cancer group(P<0.05～0.01),but there was no significant difference between IM +ATP  group,IMⅡb group and cancer group(P>0.05).Conclusion There are similar epithelial cell ultrastructure and molecular biological changes in ATP ,IMⅡb and cancer,hence there are similar patterns of occurrence,development and reverse,which contributes to the prevention and cure.

关 键 词：肠化生 不典型增生 超微结构 微量元素 血清过氧化脂质 过氧化物歧化酶 脱氧核糖核酸 慢性胃炎 

分 类 号：R573.3[医药卫生—消化系统]