唾液富酪蛋白对致龋细菌粘附的影响  

The effects of human salivary statherin on adherence of cariogenic bacteria to hydroxyapatite in vitro

作　　者：候建霞[1] 王强[1] 黄瑞哲[1] 阮梅生[1] 景娟[1] 

机构地区：[1]西安交通大学口腔医学院

出　　处：《实用口腔医学杂志》2001年第5期374-376,共3页Journal of Practical Stomatology

基　　金：陕西省自然科学基金资助 (98SM50 )

摘　　要：目的 :探讨唾液富酪蛋白 ( statherin)对致龋细菌变形链球菌和血链球菌粘附的影响。方法 :用高效疏水色谱分离提纯 3 0名个体唾液富酪蛋白并观察不同浓度唾液富酪蛋白与变形链球菌和血链球菌粘附的关系。结果 :唾液富酪蛋白明显促进变形链球菌对羟磷灰石的粘附且其促进作用的大小与其浓度有关( P<0 .0 5 ) ;而对血链球菌的粘附无明显影响 ( P>0 .0 5 )。结论 :唾液富酪蛋白可促进变形链球粘附于羟磷灰石上。砄bjective:To investigate the effects of salivary statherin on adherence of two kinds of main cariogenic bacteria. Methods: Human whole salivary statherin was separated and purified by high performance hydrophobic interaction chromatography (HPHIC) and was further identified by SDS PAGE electrophoresis and amino acid analysis.Then the adherence of Streptococcus mutans ( S.mutans serotype c,7H) and Sreptococcus sanguis (S.sanguis ,ATCC 10557)to hydroxyapatite (HA), which was covered with purified statherin or whole saliva (positive control) or PBS buffer (negative control) as experimental pellicles respectively,was studied by bacteria counting.Results:①More S.sanguis adhered to the experimental pellicles than S.mutans ( P <0.05).②The adherence of S.mutans to HA was promoted by statherin in a dose dependant manner ( P <0.05), while that of S.sanguis was not. Conclusion:Salivary statherin may promote the adherence of S.mutans to HA.

关 键 词：唾液 富酷蛋白 致龋细菌 粘附 变形链球菌 血链球菌 羟基磷灰石类 

分 类 号：R780.2[医药卫生—口腔医学]