扫描同聚焦显微镜观察血链菌和粘性放线菌生物膜的形成  

Streptococcus sanguis and Actinomycetes viscosus in oral biofilm foromation observed by sanning confocal laser microscopy

作　　者：李鸣宇[1] 刘正[1] 朱彩莲[1] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔研究所,200011

出　　处：《实用口腔医学杂志》2001年第5期425-427,共3页Journal of Practical Stomatology

基　　金：上海市教委基金资助 (97B1 7)

摘　　要：目的 :观察血链菌和粘放菌体外形成生物膜的动态过程。方法 :采用同聚焦显微镜技术 ,原位、立体、动态观察血链菌和粘放菌体外形成的生物膜。结果 :生物膜形成 8h厚度达 15 .4 μm,细菌已聚集成簇 ,菌细胞分布是表层和基底较少 ,中间层较厚并较密。生物膜形成 16h后已基本成熟 ,细菌成簇厚度增加达 3 4 .3 μm。 4 8h后生物膜已成熟成谷穗状。结论 :血链菌和粘放菌具有相互作用促进体外形成生物膜。 CL砄bjective:To observe streptococcus sanguis (S.s) and Actinomycetes viscosus (A.v) in oral plaque biofilm formation.Methods:20 ml of saliva obtained from a health adult was centrifuged at 4 ℃ and 10 000 r/min for 10 min.The supernatant was disinfected in 60 ℃ water bath for 30 min.Glass coverslips in the size of 24 mm×24 mm were immersed into the saliva supernatant for 2 h to obtain biofilm.100 μl of S.s ATCC 34 and A.v ATCC 19246 mixture cultured in TSB at the density of 10 5～6 CFU/ml was added into 20 ml of TSB,and then,the coverslips with biofilm were put into the mixture.The biofilm and bacteria were observed by scanning confocal laser microscopy at various times.Resuts:The biofilm reached the thickness of 15.4 μm in 8 h and the clumps of the bacteria were mostly in the midle layer of the biofilm.The biofilm increased to 34.3 μm in 16 h and became tassle like in 48 h.Conclusion: S.s and A.v may play some roles in the oral biofilm formation.

关 键 词：扫描同聚焦显微镜 血链菌 粘性放线菌 生物膜 龋病 

分 类 号：R780.2[医药卫生—口腔医学]