1,25-二羟基维生素D_3诱导HL-60细胞分化并下调nm23基因的表达  

Cell differentiation and down regulation of nm23 gene expression in HL-60 cells induced by 1,25 dihydroxyvitamin D_3

作　　者：朱宁希[1] 吕庆华[2] 周莹[1] 虞荣喜[1] 

机构地区：[1]杭州浙江省中医院,310006 [2]浙江大学肿瘤研究所

出　　处：《中华血液学杂志》2001年第12期639-641,共3页Chinese Journal of Hematology

摘　　要：目的　观察 1,2 5 二羟基维生素D3 [1,2 5 (OH) 2 D3 ]对白血病细胞株HL 6 0的诱导分化作用并探讨其机制。方法　通过MTT试验、细胞形态观察、表面标志分析和硝基四氮唑蓝 (NBT)还原反应 ,观察 1,2 5 (OH) 2 D3 作用后HL 6 0细胞增殖的改变和分化情况 ;通过流式细胞仪和RT PCR检测细胞周期和nm2 3基因表达的变化。结果　HL 6 0细胞经 1,2 5 (OH) 2 D3 作用后增殖受抑制、向成熟单核细胞系分化、细胞被阻滞在G1 期、nm2 3基因的表达下调。结论　 1,2 5 (OH) 2 D3 对HL 6 0细胞株具有诱导分化作用 ,其作用机制可能与下调nm2 3基因的表达有关。Objective To investigate the differentiation induction effect of 1,25 dihydroxyvitamin D 3 on leukemia cell line HL 60 and its mechanisms. Methods The changes of proliferation rate and differentiation in HL 60 cells treated with 1,25 dihydroxyvitamin D 3 were observed by MTT test, surface marker analysis and NBT reduction reaction, the effects on cell cycle and nm23 gene expression by flow cytometry and RT PCR. Results After exposing to 1,25 dihydroxyvitamin D 3, the proliferation was inhibited and the cells were induced to differentiate towards more mature monocytes. During differentiation, HL 60 cells were arrested in G1 phase and the expression of nm23 gene was down regulated. Conclusion 1,25 dihydroxyvitamin D 3 could induce differentiation of HL 60 cells, this effect might be related to the down regulation of nm23 gene expression.

关 键 词：1 25-二羟基维生素D3 HL-60细胞系 细胞分化 NM23基因 基因表达 白血病 

分 类 号：R73-3[医药卫生—肿瘤]