早期肝癌中端粒酶活化与细胞增殖相关性研究  

THE EXPRESSIONS OF TELOMERASE PROTEIN hTRT GENE?PCNA AND THEIR RELATIONSHIP IN SMALL HEPATOCELLULAR CARCINOMA(SHC)

作　　者：陆连英[1] 王桂兰[1] 鄂群[2] 

机构地区：[1]江苏省海安县人民医院病理科 [2]南通医学院病理解剖学教研室,海安226600

出　　处：《南通医学院学报》2001年第4期357-358,共2页ACTA Academiae Medicinae Nantong

摘　　要：目的 :研究早期肝癌中端粒酶基因蛋白 (h TRT)与细胞增殖核抗原 (PCNA)表达及其相关性。方法 :在2 0例早期肝癌中用原位杂交检测 h TRT基因 ,免疫组化方法检测 PCNA的表达。结果 :h TRT阳性信号检出率 80 .0 % ,PCNA强阳性率为 5 5 .0 % ;癌中 h TRT阳性信号、PCNA表达强度显著高于癌旁非癌组织 (P<0 .0 5 ) ;癌不同分化组中 PCNA表达有显著差异 (P<0 .0 5 ) ;癌中 h TRT阳性信号分布有膜型和浆型两种形式 ,其分布形式、信号强度均与 PCNA阳性强度密切相关 (P<0 .0 1)。结论 :肝癌发生与细胞增殖密切相关。端粒酶活化是肝癌发生的早期事件 ,是肝细胞增生和恶性转化所必需 ,其检出结果对鉴别肿瘤恶变有重要意义。Objective:To study the expressions of telomerase protein hTRT gene and PCNA and their significance in SHC.Methods: In situ hybridization (ISH) and immunohistochemistry technique were used to investigate the expression of telomerase protein hTRT gene and PCNA expressions in 20 cases of SHC. Results:The expressions of telomerase protein hTRT gene ,PCNA in SHC were 80%,and 55%, respectively. The expressions of hTRT and PCNA stronger expression in SHC were higher than that in paracarcinoma tissues (P<0.05) .There was a significant difference in the expressions of PCNA in the different differentiated groups (P<0.05). Two types of hTRT distribution patterns were found, membrance type and cytoplasmic type ,in which patterns were positively related with the stronger expression of PCNA .Conclusion: Cellular proliferation was one of the basement of carceration . Telomerase protein hTRT gene activity may play an important role in the multisteps course of SHC tumorgenesis and it was early event in SHC, and considered necessary for liver cell proliferation and malignant transformation, and the detection of hTRT was valuable to clinical oncology diagnosis.

关 键 词：早期肝癌 端粒酶蛋白hTRT基因 原位杂交 细胞增殖核抗原 肝肿瘤 

分 类 号：R735.7[医药卫生—肿瘤]