缺氧缺血时新生鼠脑内t-PA活性的变化  

Activity of Tissue-type Plasminogen Activator in Rat Brain on Hypoxia

作　　者：王玮[1] 徐剑文[2] 陈春鹏[2] 

机构地区：[1]福建医科大学神经生物学研究中心,福州350004 [2]福建医科大学人体解剖学与组织胚胎学系,福州350004

出　　处：《福建医科大学学报》2001年第4期315-317,共3页Journal of Fujian Medical University

基　　金：福建省自然科学基金资助项目 (C970 42 )

摘　　要：目的　探讨缺氧缺血时新生鼠脑不同源性组织型纤溶酶原激活物 (t- PA )活性的变化及其临床意义。　方法　一日龄新生 SD大鼠分为空白对照组、假手术组和 3个缺氧和复氧时间不同的实验组 (n=4 ) ;体外培养鼠脑微血管内皮细胞和星形胶质细胞 ,并分为空白对照组和缺氧组 ;取鼠脑组织和培养液测 t- PA活性。　结果　在动物组中以缺氧缺血组的 t- PA活性最高 ,随着复氧时间的增加而下降 (P<0 .0 1) ;缺氧时鼠脑微血管内皮细胞 t-PA活性增高 ,而星形胶质细胞 t- PA活性无变化。　结论　缺氧导致新生鼠脑内 t- PA活性增高 ;随着缺氧改善 ,t-PA活性在一定程度上可以下降 ;缺氧时新生鼠脑内可能主要是微血管内皮细胞产生的 t- PA活性增高 ,降解微血管基膜 ,破坏微血管的完整性 。Objective\ To investigate variation in activity of the tissue\|type plasminogen activator(t\|PA) in rat brain on hypoxia and its clinical significance.\ Methods\ Neonatal SD rats were divided \{into\}: (1)control group, (2)false operation group, (3)hypoxic\|ischemia group, (4)surviving for 24 hours after hypoxic\|ischemia group, (5)surviving for 48 hours after hypoxic\|ischemia group,n=4.\ Brain microvascular endothelial cells and astrocytes were cultured in vitro in control and hypoxia group.\ Cerebral tissues and cultural fluids were measured for activity of t\|PA.\ Results\ The activity of t\|PA in hypoxic\|ischemic group was the highest in hypoxic\|ischemic group, and came down in the surviving.\ However, \{t\|PA\} activity of endothelial cells was enhanced, and astrocytes did not changed in hypoxia group in vitro.\ Conclusion\ Hypoxia can stimulate to raise the activity of t\|PA in the brain of neonatal rat, and t\|PA activity comes down as surviving.\ The t\|PA activity of microvascular endothelial cells rises in brain on hypoxia condition.\ It damages the basement membrane of cerebral microvessels and cerebral hemorrhage may occur finally.

关 键 词：脑缺氧 脑缺血 纤溶酶原激活物 动物实验 

分 类 号：R363[医药卫生—病理学]