亚低温联合黄芪对大鼠局灶脑缺血再灌注损伤的神经保护作用  

The neuroprotective effects of mild hypothermia plus Astragalus in a model of cerebral artery occlusion in rat

作　　者：梅志忠[1] 李承晏[1] 吴国祥[1] 喻文莉[1] 

机构地区：[1]武汉大学人民医院神经内科,硕士研究生430060

出　　处：《卒中与神经疾病》2001年第5期275-277,共3页Stroke and Nervous Diseases

摘　　要：目的　探讨亚低温 (32± 1℃ )联合黄芪对大鼠局灶脑缺血再灌注损伤的协同保护作用。方法　采用改良线栓法制备大鼠大脑中动脉闭塞再灌注模型 ,将 2 4只SD大鼠随机分为四组 (每组 6只 ) :常温组 ;亚低温组 ,再灌注后立即诱导亚低温并持续 12小时 ;黄芪组 ,脑缺血后立即按 1g/kg剂量腹腔注射黄芪 ;黄芪加亚低温组 ,脑缺血后立即按 1g/kg剂量腹腔注射黄芪 ,再灌注后立即诱导亚低温并持续 12小时。每组均脑缺血 6小时后再灌注。观察脑梗死体积、神经功能缺损评分和病理改变。结果　黄芪加亚低温组脑梗死体积为 112 .41± 16 .6 4mm3 ,亚低温组为 146 .6 8± 14.88mm3 ,经两因素方差分析有显著性意义 (P <0 .0 5 )。结论　亚低温联合黄芪对脑缺血再灌注损伤具有协同保护作用。Objective To investigate the neuroprotective effects of mild hypothermia plus Astrsgalus in the cerebral middle artery occlusion in rat.Methods All SD rats underwent 6 hours of middle artery occlusion followed by reperfusion. The nomothermia group ( n =6). The mild hypothermic group ( n =6): The mild hypothermia (32±1℃) were induced after reperfusion and lasted for 12 hours. Astragalus treatment group ( n =6): The Astragalus (1 mg/kg) was injected into peritoneal cavity immediately after ischemia. The Astragalus plus mild hypothermia group ( n =6): mild hypothermia was established after reperfusion, which continued for 12 hours and was injected immediately after ischemia. The infarct volume and histologic changes were observed.Results The infact volumes were 112.41 ± 16.64 mm 3 in the astragalus plus mild hypothermic group and 146.68 ± 14.88 mm 3 in the mild hypothermic group. The infarct volume was smaller in rats treated by the Astragalus plus hypothermia than in those treated with the mild hypothermia ( P < 0.05 ). Conclusion The study suggest that synergetic effect of the mild hypothermia and Astragalus should be obvious.

关 键 词：脑缺血 再灌注 亚低温 黄芪 脑梗死体积 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R282.710.7[医药卫生—临床医学]