维生素C增强氧化砷联用二甲萘醌诱导的U937细胞凋亡及其机制  

Ascorbic acid enhances the apoptosis of U937 cells induced by arsenic trioxide in combination with DMNQ and its mechanism

作　　者：高飞[1] 易静[1] 史桂英[1] 李慧[1] 石学耕[1] 王志伟[1] 汤雪明[1] 

机构地区：[1]上海第二医科大学细胞生物学教研室,200025

出　　处：《中华血液学杂志》2002年第1期9-11,共3页Chinese Journal of Hematology

基　　金：国家自然科学基金资助项目 ( 39730 2 70 );上海市教委高校科技发展基金资助项目 ( 2 0 0 0B10 1)

摘　　要：目的　探讨维生素C是否可增强三氧化二砷 (As2 O3 )联用二甲萘醌 (DMNQ)对白血病细胞株U937的促凋亡效应及其机制。方法　以As2 O3 、维生素C、DMNQ不同组合孵育细胞 ,采用流式细胞仪及电镜检测细胞凋亡 ,并设立各种对照。结果　维生素C可增加As2 O3 联用DMNQ(10 μmol/L)诱导U937细胞发生凋亡的比例 (不加和加用维生素C细胞凋亡率分别为 35 .2 4%和 6 1.2 0 % ) ;过氧化氢酶可逆转这一效应 ;维生素C对DMNQ(2 0 μmol/L)单独诱导U937细胞凋亡无影响。 结论　维生素C在As2 O3 存在的情况下 ,通过活性氧依赖的途径 。Objective To investigate whether ascorbic acid could enhance the efficacy of arsenic trioxide (As 2O 3) combined with 2,3 dimethoxy 1,4 naphthoquinone(DMNQ) in inducing the apoptosis of leukemia cell line U937 and its possible mechanism. Methods Flow cytometry and electron microscopy were applied to detect apoptosis of U937 cells after treatment with various combinations of As 2O 3, DMNQ and ascorbic acid for 24 hours. Results As 2O 3 and DMNQ induced apoptosis of U937 cells was enhanced (35.24%→61.20%) upon cotreatment with ascorbic acid. Catalase could reverse this effect of DMNQ. Ascorbic acid had no effect on DMNQ induced apoptosis of U937 cells. Conclusion Ascorbic acid enhanced the apoptosis of U937 cells via reactive oxygen species dependent pathway in the presence of As 2O 3.

关 键 词：砷剂 抗坏血酸 细胞凋亡 活性氧组分 维生素C 

分 类 号：R733.71[医药卫生—肿瘤]