间期荧光原位杂交技术检测急性粒-单核细胞白血病inv(16)  

Detection of inv(16) in acute myelomonocytic leukemia by interphase fluorescence in situ hybridization

作　　者：李建勇[1,2] 潘金兰[1] 吴亚芳[1] 过宇[1] 薛永权[1] 

机构地区：[1]苏州大学附属第一医院,江苏省血液研究所,现在215006 [2]南京医科大学第一附属医院,江苏省人民医院血液科

出　　处：《中华血液学杂志》2002年第1期30-32,共3页Chinese Journal of Hematology

摘　　要：目的　探讨荧光原位杂交 (FISH)技术在检测inv(16 ) (p13q2 2 )中的价值。方法　采用红色荧光素SpectrumRed标记的酵母人工染色体 (YAC)克隆 85 4E2 [跨越第 16号染色体短臂inv(16 )断裂点 ]作为探针 ,用单色间期FISH检测 2 6例急性粒 单核细胞白血病 (AML M4 )inv(16 ) ,并与经典细胞遗传学结果进行比较分析。结果　采用R显带分析的 2 5例患者均未发现inv(16 ) ,1例G显带分析的M4Eo患者伴inv(16 ) ;FISH分析显示 9例inv(16 ) ,其中 3例M4Eo均伴有inv(16 )。在 9例inv(16 )患者中 ,特征性的 3个红色荧光信号的阳性率仅为 13.3%～ 32 .1% (中位数 2 1.3% )。结论　YAC 85 4E2和间期FISH是检测inv(16 )Objective To evaluate fluorescence in situ hybridization (FISH) in the detection of inv(16)(p13;q22). Methods Spectrum red labeled yeast artificial chromosome (YAC) clone 854E2 which spans the breakpoint cluster region in MYH11 in band 16p13 and single color interphase FISH were used to detect inv(16) in 26 cases of acute myelomonocytic leukemias (AML M 4), and the results were compared with that of conventional cytogenetic analysis. Results R banding karyotyping test revealed no inv(16) in 25 cases, one AML M 4Eo case showed inv(16) by G banding. Nine cases including all three M 4Eo had inv(16) by FISH analysis, among whom the characteristic fluorescence signal pattern of the inv(16) was seen in 13.3% to 32.1%(median,21.3%) of the tested cells. Conclusion YAC 854E2 and interphase FISH provide a powerful technique in the detection of inv(16)(p13q22).

关 键 词：白血病 粒-单核细胞 急性 荧光原位杂交 酵母人工染色体 

分 类 号：R733.7[医药卫生—肿瘤]