蛋白酶体抑制剂对白血病细胞系细胞周期的影响  

Effect of proteasome inhibitor on cell cycle progression of leukemic cell lines

作　　者：兰雨[1] 张学敏[2] 杨平地[3] 胡美茹[4] 杨怡[2] 沈倍奋[4] 

机构地区：[1]第二军医大学研究生队,上海200433 [2]国家生物医学分析中心,北京100850 [3]海军总医院血液科,北京100037 [4]军事医学科学院三所四室,北京100850

出　　处：《基础医学与临床》2002年第1期40-42,共3页Basic and Clinical Medicine

基　　金：国家自然科学基金 (30 0 70 377)

摘　　要：近年来的研究显示蛋白酶体抑制剂可诱导多种人肿瘤细胞系的凋亡 ,机制之一是其对细胞周期的影响。本实验就蛋白酶体抑制剂Z LLL CHO对白血病细胞系M 0 7e、TF 1、HL 60细胞周期的影响进行了初步研究。应用MTT法显示Z LLL CHO对三株白血病细胞系呈不同程度的细胞杀伤效应。流式细胞仪检测显示应用 2 .5 μmol LZ LLL CHO 8h后可使G2 M期细胞比例增加 ,此种效应在S +G2 M期比例最高的HL 60中最为显著。对凋亡峰的考察发现 ,Z LLL CHO作用后可使细胞凋亡峰的比例增加 ,但与其对细胞周期的影响程度无明显相关性。上述结果提示蛋白酶体抑制剂作用后可使白血病细胞系G2 M期细胞比例增加 ,细胞对其诱导凋亡的敏感性与细胞周期受影响的程度无明显相关性 ,提示其他的相关机制参与了蛋白酶体抑制剂诱导白血病细胞凋亡的调控。Proteasome inhibitors have been shown to induce apoptosis of human malignant cells One of the mechanisms is its effect on cell cycle. In this study, the effect of the proteasome inhibitor Z LLL CHO on cell cycle progression of leukemic cell lines M 07e?TF 1 and HL 60 was investigated MTT assay showed that the cytotoxic effects of Z LLL CHO on the three cell lines varied from each another Accumulation of cells in the G 2/M phase of the cell cycle was shown by FACS analysis after exposing the cells to 2 5μmol/L Z LLL CHO for 8h This effect was most evident in HL 60 which had the highest percentage of cells in the S+G 2/M phase Increasing of sub G 0/G 1 fraction was detected after Z LLL CHO treatment, which did not correspond with the effect that Z LLL CHO put on the cell cycle progression In summary, exposure of leukemic cell lines to proteasome inhibitor Z LLL CHO caused them to accumulate at the G 2/M phase of the cell cycle The sensitivity of cells to undergo apoptosis was out of accord with the change of the cell cycle progression The result suggested some mechanisms that might be involved in the regulation of apoptosis induced by proteasome inhibitor

关 键 词：蛋白酶体抑制剂 白血病细胞系 细胞凋亡 细胞周期 

分 类 号：R733.7[医药卫生—肿瘤]