褪黑素对抗小鼠束缚应激血清免疫抑制活性  

Melatonin counteracts the immunosuppressive effect of serum from stressed mice on lymphocyte proliferation

作　　者：杨春星[1] 汪静雪[1] 许晓楠[1] 范少光[1] 梅林[1] 

机构地区：[1]北京大学医学部生理学系,北京100083

出　　处：《基础医学与临床》2002年第1期84-87,共4页Basic and Clinical Medicine

摘　　要：为了阐明褪黑素对免疫功能的调节作用 ,本文利用束缚应激模型 ,观察褪黑素在整体和离体水平是否能够对抗应激血清抑制正常淋巴细胞转化的活性。结果表明 ,腹腔注射褪黑素 1 2 5～ 50 0mg kg,能够剂量依赖性地对抗应激血清对正常淋巴细胞转化的活性 ;在体外培养中 ,1 0 -1 4 ～ 1 0 -5mol L褪黑素对正常淋巴细胞转化无任何作用 ,生理浓度的褪黑素也不能对抗应激血清对正常淋巴细胞转化的抑制作用。上述结果初步提示 :褪黑素对应激血清免疫抑制活性的抑制作用可能不是发生在靶 (淋巴 )细胞水平 ,而是通过药理学机制在束缚应激的早期发挥作用。The pineal neurohormone, melatonin (MLT), exerts an important immunoregulation on the mammalian immune system. We found that exogenous MLT counteracts the immunosuppressive effect of serum from mice subjected to physical restraint. In the experiment, phosphate buffered saline (PBS) control, PBS stress, MLT control and MLT stress groups were tested. Mice from MLT stress group were injected with MLT (12.5～50mg/kg, i.p.) for 2 days. On the 2nd day, after MLT injection, the mice were stressed by continued restraint for 16 hours as did before. Serum samples were tested for their immunosuppressive effect on normal lymphocyte transformation. The results showed that the lymphocyte transformation (counts/min) in PBS stress group was inhibited ( P <0.01 vs PBS control, ANOVA); whereas in MLT stress group, the cpm was significantly higher than in PBS stress group ( P <0.01, ANOVA), but MLT itself did not increase counts/min of normal lymphocyte transformation as showed in MLT control group and in vitro lymphocyte culture. Moreover, MLT could counteract the suppressive effect of stressed serum in a dose dependent manner. As shown by in vitro test, the possibility of MLT functioned with a physiological mechanism and functioned at the target cell (lymphocyte) level was ruled out. In conclusion: MLT could counteract the immunosuppressive effect of stressed serum with a pharmacological mechanism, which may happen in the early stage of restraint stress.

关 键 词：褪黑素 束缚应激 淋巴细胞转化 免疫抑制活性 

分 类 号：R335[医药卫生—人体生理学]