高效液相色谱法测定人血浆中缬沙坦的药物浓度  

Determination of Valsartan Concentration in Human Plasma With HPLC

作　　者：刘红梅[1] 刘艳[1] 金锐[1] 王珍[1] 孙考祥[1] 陆义诚[1] 

机构地区：[1]哈尔滨医科大学附属二院药学部,哈尔滨市150086

出　　处：《中国药房》2001年第7期414-415,共2页China Pharmacy

摘　　要：目的 :测定人血浆中缬沙坦的药物浓度。方法 :采用液固萃取法提取 ,高效液相色谱法测定。色谱柱 :HypersilODSC18(4 6nm×200nm ,5μm ) ;流动相 :乙腈 -0 01mol/L的磷酸盐缓冲液 (50∶50 ,pH2 8) ;流速 :1 5ml/min ;荧光检测器 :λex为265nm ,λem为378nm。结果 :缬沙坦的保留时间为5 4min ,定量线性范围0 05～5μg/ml,方法回收率>90 % (n=5) ,日内、日间RSD<10 % (n=5)。结论 :本法快速 ,定量准确 。OBJECTIVE:To determine the valsartan concentration in human plasma METHODS:The plasma sample was extracted with a liquid-solid method and determined with HPLC,stationary phase was Hypensil ODS C18(4 6nm×200nm,5μm),mobile phase consisted of acetonitrile and 0 01mol/L KH2PO4 buffer(pH 2 8)(50∶50) The flow rate was 1 5ml/min Detection was performed with fluorescence detector at λex 265nm,λem 378nm RESULTS:The retention time of valsartan was about 5 4 minutes,and the linear range of quantity was 0 05～5μg/ml The recoveries of methodology were more than 90%(n=5) Inter-day and intra-day RSD were less then 10%(n=5) CONCLUSION:This method is rapid and accurate It can be applied to determining the plasma valsartan concentration and studying on pharmacokinetics

关 键 词：缬沙坦 血药浓度 高效液相色谱法 

分 类 号：R914.1[医药卫生—药物化学] R969.1[医药卫生—药学]