剪切力改变后动脉内皮细胞通透性的变化  

Permeability change in arterial endothelium under shear stress

作　　者：舒强[1] 金鑫[2] 凌光烈[1] 王速[3] 

机构地区：[1]中国医科大学第一附属医院外科局解教研室,辽宁沈阳110001 [2]中国医科大学基础医学院药理教研室,辽宁沈阳110001 [3]中国医科大学实验病理研究室,辽宁沈阳110001

出　　处：《中国病理生理杂志》2002年第1期24-27,共4页Chinese Journal of Pathophysiology

基　　金：留学回国人员科研启动基金资助项目 (教外留字 1999747号 )

摘　　要：目的 :研究剪切力改变后内皮通透性及其超微结构。方法 :饲以普通或高脂肪食的兔腹主动脉狭窄 6 0 7% ,高速摄像微粒子示踪技术血流分析 ,依万斯蓝和苏丹Ⅳ动脉染色 ,辣根过氧化物酶法电镜观察。结果 :距狭窄近侧 1mm的动脉前、后壁剪切力分别为 74dyn/cm2 和 317dyn/cm2 ,为高剪切力区 ;狭窄远侧 3mm前、后壁分别为 - 18dyn/cm2 和 - 71dyn/cm2 ,为返流、涡流、湍流和停滞的低剪切力区。狭窄近、远段内皮通透性均增高 ,在蓝染或苏丹Ⅳ阳性区 ,狭窄远段内皮细胞连接部完全开放型的百分率 (88 5 %或 88 1% )明显大于近段 (2 2 7%或 30 % )(P <0 0 1) ,远段标记HRP小胞的密度 (2 5 7± 1 14)× 10 12 /m2 或 (2 72± 1 81)× 10 12 /m2 明显高于近段 (1 2 4± 1 0 6 )× 10 12 /m2 或 (1 90± 1 47)× 10 12 /m2 (P <0 0 1)。结论 :低剪切力更易导致内皮细胞对大分子物质通透性增高和动脉硬化形成。AIM: To study the endothelial permeability and its ultrastructures under different shear stress. METHODS: ① Abdominal aortas of rabbits fed with ordinary and high fat diets were stenosed to 60.7% by operation. ② Blood flow analysis with microsphere tracing technique with high-speed vediocamera was conducted. ③ Arterial staining with Evans blue and Sudan Ⅳ. ④ Electron microscopy with horseradish peroxidase (HRP) were quantitatively analyzed to determine the permability of arterial endothelium. RESULTS: Shear stresses of anterior and posterior arterial walls at 1 mm proximal to the stenosis were 74 dyn/cm 2 and 317 dyn/cm 2 respectively,as high shears stress region. Those at 3 mm distal to the stenosis were -18 dyn/cm 2 and -71 dyn/cm 2 respectively, as low shear stress region with retrograde, vortex, turbulent flow and stagnation. The endothelial permeability increased in both sides of proximal and distal segments of stenosis. In the Evans blue or Sudan Ⅳ positive areas, the percentages (88.5% or 88.1%) of complete-open type of interendothelial junctions in the distal segments were larger than those (22.7% or 30%) in the proximal ones, respectively,( P< 0.01). The densities [(2.57±1.14)×10 12 /m 2 or (2.72±1.81)×10 12 /m 2] of vesicles labeled with HRP in the distal segments were significantly higher than those [(1.24±1.06)×10 12 /m 2 or (1.90±1.47)×10 12 /m 2) in the proximal segments, respectively ( P< 0.01). [WTHZ]CONCLUSION: Low shear stress induces readily the increase in endothelial permeability for macromolecules and atherosclerosis development.

关 键 词：渗透性 血液动力学 剪切力 动脉 内皮细胞 

分 类 号：R364.1[医药卫生—病理学]