鼠尾胶原及C-erbB-2蛋白抗体对肝癌(HepG-2)细胞粘连和增殖的影响  

Effects of rat tail collagen and C-erbB-2 antibody on the adhesion and proliferation in hepatocellular carcinoma cell

作　　者：张海伟[1] 罗英儒[2] 陈小琳[3] 郑佩娥[1] 司徒锐[1] 

机构地区：[1]暨南大学医学院病理教研室,广东广州510632 [2]暨南大学医学院药理教研室,广东广州510632 [3]暨南大学医学院病理生理教研室,广东广州510632

出　　处：《中国病理生理杂志》2002年第1期59-61,共3页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金 (No .395 0 0 0 5 6 );广东省"五个一兴教工程"基金资助

摘　　要：目的 :揭示胶原及C -erbB - 2蛋白抗体对肝癌细胞粘连和增殖的影响 ,提供抗癌治疗的线索。方法 :以人肝癌细胞细胞系 (HepG - 2 )为靶子 ,利用C -erbB - 2抗体及鼠尾胶原体外作用于靶细胞 ,观察C -erbB - 2抗体及鼠尾胶原对肝癌细胞粘连和增殖的影响。结果 :鼠尾胶原有加强肝癌细胞粘附作用而对其增殖作用无显著影响 ;C -erbB - 2抗体能明显抑制其细胞的增殖和粘连 ,也明显拮抗鼠尾胶原对其细胞粘连性的加强作用。结论 :人肝癌细胞HepG - 2的粘连和增殖与胶原的作用存在与否有关 ,这与C -erbB - 2基因蛋白介导的信号传递作用存在着密切的关联 ,阻断C -erbB - 2基因的信号传递作用对肝癌治疗是一个有前景的战略性靶点。AIM: To explore the effect of collagen and C-erbB-2 protein on the adhesion and the proliferation in hepatocellular carcinoma cells.METHODS: Hepatocellular carcinoma cell line(HepG-2) identified to positive for C-erbB-2 gene was used to study the adhesion and the growth feature by the action of rat tail collagen and C-erbB-2 antibody.RESULTS: The action of rat tail collagen to potentiated the adhesion in HepG-2 cells was significantly but no proliferation effect was observed. C-erbB-2 antibody inhibited the adhesion and proliferation of HepG-2 cells and also abolished the potentiated effect of rat tail collagen on the adhesion in HepG-2 cells.CONCLUSIONS: The signaling transduction mediated by C-erbB-2 protein was correlated to the adhesion and the proliferation of HepG-2. The blockage of C-erbB-2 gene signal transduction may be a strategic target to the treatment of liver cancer in the future.

关 键 词：肝细胞瘤 细胞粘连 细胞增殖 C-ERBB-2蛋白 

分 类 号：R735.7[医药卫生—肿瘤]