聚甘氨酸修饰碳纤维微电极差示脉冲伏安法测定尿酸  

Determination of Uric Acid by Different Pulse Voltammetry Using Poly-Glycine Modified Carbon Fiber Microelectrode

作　　者：刘灵芳[1] 王翠红[1] 张娟[1] 冶保献[1] 

机构地区：[1]郑州大学化学系,郑州450052

出　　处：《天津师范大学学报（自然科学版）》2001年第4期28-33,共6页Journal of Tianjin Normal University：Natural Science Edition

基　　金：河南省杰出青年基金项目 ( 992 4);教育部<高等学校骨干教师资助计划>资助项目 ( 1988-2 0 0 1)

摘　　要：研究聚甘氨酸修饰碳纤维微电极 (CFME)的电化学性质和在抗坏血酸存在下测定尿酸的分析方法 ,以及尿酸在该聚合膜电极上反应的动力学参数 .在选定的条件下 ,尿酸和抗坏血酸的峰电位分别为 0 .3 3 V和 0 .0 3 V,两峰电位差达 3 0 0 m V,可以有效地避免抗坏血酸对尿酸测定的干扰 .In this paper, we present a new method for determination of uric acid in the presence of high concentration of vitamin C by different pulse voltammetry using poly glycine modified carbon microelectrode. The electrode reaction dynamics parameters were investigated by electrochemical methods. Under the selective condition, the peak potentials of uric acid and ascorbic acid are 0. 33 V and 0.03 V respectively. Based on this point, we have established a method for determining uric acid and abstaining the interference of ascorbic acid. This method has been used in determining of trace uric acid in urine sample with satisfactory results.

关 键 词：化学修饰微电极 聚甘氨酸 尿酸 抗坏血酸 碳纤维 测定 电化学伏安法 嘌呤代谢 

分 类 号：R446.12[医药卫生—诊断学] Q592.2[医药卫生—临床医学]