封闭Her-2蛋白拮抗碱性成纤维细胞生长因子对人肝癌细胞的粘连效应  

Effect of basic fibroblast growth factor counteracted by blockage on adhesion of Her-2 protein of human hepatocellular carcinoma cell

作　　者：罗英儒[1] 张海伟[2] 王玉萍[3] 夏克勤[4] 

机构地区：[1]暨南大学药学院药理学教研室 [2]暨南大学医学院病理学教研室 [3]暨南大学医学院第一附属医院理疗科 [4]暨南大学医学院,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2001年第6期27-30,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金 (395 0 0 0 5 6 );广东省"五个一兴教工程"基金资助

摘　　要：目的 :探索碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor ,bFGF)影响癌细胞粘连的信号传递途径 ,提供抗癌治疗的线索。方法 :以人肝癌细胞系 (HepG2 )为靶子 ,明确靶细胞存在Her-2蛋白表达的同时 ,利用Her- 2蛋白抗体封闭靶细胞Her - 2蛋白来研究bFGF作为配体与否的细胞粘连效应性。结果 :靶细胞HepG2确实具有Her- 2蛋白表达 ;利用抗体封闭靶细胞Her - 2蛋白时 ,靶细胞明显地呈现拮抗bFGF作为配体的细胞粘连加强效应。结论 :bFGF对人肝癌细胞粘连性的影响与Her- 2基因蛋白介导的信号传递作用有关。Aim: To explore the signaling transduction pathway by the effect of basic fibroblast growth factor(bFGF) on the adhesion and to provide a clue to the treatment of cancer possible. Methods: Human hepatocellular carcinoma cell line(HepG2) was used to identify the expression of Her-2 protein and to comparatively study the effect of bFGF on the adhesion of HepG2 cells by the antibody of Her-2 protein which would block the cells Her-2 protein if the cell possess Her-2 protein . Results: HepG2 cells possessed the Her-2 protein besides the adhesion potentiated by the effect of bFGF was counteracted by the blockage of Her-2 protein. Conclusion:The data suggest that the effect of bFGF on the adhesion was correlated with the signaling transduction mediated by Her-2 protein .

关 键 词：肝癌 粘连性 HER-2蛋白 碱性成纤维细胞生长因子 信号传递 治疗 

分 类 号：R735.7[医药卫生—肿瘤]