左旋四氢巴马汀在大鼠脑缺血-再灌流时对神经元凋亡的影响  

Effects of L-tetrahydropalmatine on neuronal apoptosis during cerebral ischemia/reperfusion in rats

作　　者：蒋崇慧[1] 杨光田[2] 汤彦[3] 邓普珍[2] 

机构地区：[1]广东省中山市人民医院急诊科,528400 [2]同济医科大学附属同济医院急诊科 [3]湖北省十堰市东风汽车公司总医院ICU

出　　处：《中华急诊医学杂志》2001年第6期386-388,共3页Chinese Journal of Emergency Medicine

摘　　要：目的　通过观察脑缺血再灌流后海马CA1区存活神经元数目 ,原位末端标记(TUNEL)阳性细胞数目 ,热休克蛋白 70 (HSP70 )的表达以及脑组织超微结构的变化 ,探讨左旋四氢巴马汀 (L THP)对脑缺血 再灌流损伤的保护作用的机制。方法　采用Pulsinelli法建立大鼠全脑缺血 再灌流损伤模型。观察L THP在脑缺血 再灌流的迟发性损伤阶段对海马CA1区存活神经元数目 ,TUNEL阳性细胞数目 ,HSP70的表达以及脑组织超微结构的影响。结果　L THP可增加脑缺血 再灌流后HSP70的表达 ,减少神经元凋亡的发生 ,提高神经元的存活数目。结论　L THP可减少脑缺血 再灌流神经元凋亡 ,提高神经元的存活数目 ,对脑缺血Objective The number of surviving neuron,TUNEL positive neurons,HSP70 expression and the ultrastructure of the CA 1 subfield of hippocampus were examined after cerebral ischemia/reperfusion in rats and the protective effects of L tetrahydropalmatine(L THP) were also observed.Methods Pulsinellis methods were employed to establish cerebral ischemia/reperfusion animal models.The abovementioned indices were examined with and without L THP.Results L THP could upregulate the expression of HSP70 after cerebral ischemia/reperfusion.Neuronal apoptosis after cerebral ischemia/reperfusion was decreased and the number of surviving neuron was increased by L THP.Conclusion L THP had remarkable protection on cerebral ischemia/reperfusion injury.Its mechanism might be related to decreasing neuronal apoptosis. [

关 键 词：脑缺血-再灌流损伤 凋亡 热休克蛋白70 左旋四氢巴马汀 

分 类 号：R363[医药卫生—病理学]