基质金属蛋白酶组织抑制因子mRNA及蛋白在肝硬化组织中的定位  

Tissue inhibitors of metalloproteinase-1 and -2 (TIMP-1 and TIMP-2) mRNA and antigens location in the liver of patients with cirrhosis

作　　者：谢玉梅[1] 聂青和[1] 周永兴[1] 程勇前[1] 康文臻[1] 赵玉玲[1] 

机构地区：[1]第四军医大学唐都医院,西安710038

出　　处：《中华传染病杂志》2001年第6期352-354,共3页Chinese Journal of Infectious Diseases

基　　金：中国博士后科学基金资助项目 (中博基 [1999] 10 )

摘　　要：目的　了解基质金属蛋白酶组织抑制因子TIMP 1和TIMP 2蛋白及mRNA在肝硬化组织中的表达和分布状态。方法　以抗TIMP 1和TIMP 2单克隆抗体及TIMP 1和TIMP 2cDNA探针为试剂 ,采用免疫组织化学法和原位杂交技术检测肝硬化组织中TIMP 1和TIMP 2蛋白和mRNA。结果　 40例肝硬化患者肝组织中TIMP 1和TIMP 2蛋白以及mRNA表达的阳性率为10 0 % ,TIMP 1阳性的组织TIMP 2亦为阳性 ,且TIMP 1强度高于TIMP 2 ,而正常肝组织未见阳性表达。TIMP 1和TIMP 2蛋白及mRNA表达的阳性信号均位于肝细胞胞浆中 ,细胞核中无表达。结论　肝硬化患者的肝细胞中存在TIMP 1和TIMP 2蛋白及mRNA的表达 ,其表达强度与肝组织病理改变呈正相关 ,这可能是通过抑制基质金属蛋白酶 (MMPs)的活性 ,使得细胞外基质 (ECM )降解减少而引起肝硬化。Objective To study the expression and distribution of TIMP 1 and TIMP 2 in liver tissue of cirrhosis patient and to investigate the roles and pathogenesis of TIMP 1 and TIMP 2 in liver cirrhosis. Methods TIMP 1 and TIMP 2 proteins and mRNA were detected with immunohistochemistry and in situ hybridization methods using monoclonal antibodies and cDNA probes. Results mRNA and proteins of TIMP 1 and TIMP 2 were detected in all the liver tissues from 40 liver cirrhosis patients, all in cytoplasm but not nucleus. TIMP 1 and TIMP 2 were found co exist in all samples, while TIMP 1 concentration was higher. Conclusions mRNA and protein of TIMP 1 and TIMP 2 are found in all the cirrhosis patient samples. Liver TIMP 1 and TIMP 2 concentrations increase with the progression of liver cirrhosis, decrease the degradation of extracellular matrix proteins, resulting in the initiation and the development of liver fibrosis and liver cirrhosis.

关 键 词：肝硬化 金属蛋白酶组织抑制因子 细胞外基质 免疫组织化学 原位杂交 

分 类 号：R575.2[医药卫生—消化系统]