PTX腹腔注射致SD鼠点燃癫痫模型  

Kindling model of SD rats induced by injection of PTX intraperitoneally

作　　者：田发发[1] 谢光洁[1] 杨期东[1] 吕冰清[1] 

机构地区：[1]中南大学湘雅医院神经内科,长沙410008

出　　处：《湖南医科大学学报》2001年第6期525-526,共2页Bulletin of Hunan Medical University

摘　　要：目的 :观察不同剂量印防已毒 (PTX)致SD鼠慢性点燃癫痫模型及药物对该模型的影响。方法 :采用PTX腹腔注射制作SD鼠慢性点燃模型 ,并将其分为对照组、癫痫I组 (PTX ,1 5mg·kg- 1 ·d- 1 )、癫痫II组 (PTX ,2mg- 1 ·d- 1 )和苯巴比妥钠干预组 ;观察发作情况 ,并做脑电图记录。结果 :注药第 2 0d时 ,对照组无癫痫发作 ,癫痫I组 71 %SD鼠(1 0 /1 4)完全点燃 ;癫痫II组 4只死亡、2只IV级、2只V级 ;苯巴比妥钠干预组 1 7%SD鼠 (1 /6 )完全点燃。完全点燃的SD鼠在脑电图上出现典型尖波、棘波发放。结论 :在该实验中 ,PTX腹腔注射致SD鼠点燃癫痫模型的较好剂量是 1 5mg·kg- 1 ·d- 1 。Objective To observe the kindling model of Sprague Dawley (SD) rats induced by injection of different doses of picrotoxin (PTX) intraperitoneally, and to evaluate the effect of the drug on the model. Methods SD rats were devided randomly into four roups: control group, epilepsy Group I (PTX, 1.5?mg·kg -1 ·d -1 ),epilepsy Group II (PTX, 2?mg·kg -1 ·d -1 ) and the phenobarbital group. The kindling model of rats was induced by daily injection of PTX intraperitoneally. The seizures in the rats were observed, and the results of electroencephalography (EEG) were recorded. Results On the 20th day after injection of PTX, 71% SD rats were kindled in epilepsy Group I; 4 rats were dead, 2 rats were at stage IV of convulsions, and 2 rats were at stage V of convulsions in epilepsy Group II; 17% rats were kindled in the phenobarbital group. The EEG demonstrated spike discharges in the kindled rats. Conclusions In this models, the better dose of PTX is 1.5?mg·kg -1 ·d -1 , and phenobarbital sodium can protect SD rats injected PTX intraperitoneally from seizure.

关 键 词：印防已毒 疾病模型 苯巴比妥钠 大鼠 癫痫 

分 类 号：R742.1[医药卫生—神经病学与精神病学]