端粒酶反义寡聚脱氧核苷酸诱导人肝癌细胞凋亡模型构建的实验研究  

The Effect of Telomerase Antisense Oligodeoxynucleotides on the Growth of Hepatoma Cell Line

作　　者：许兰涛[1] 

机构地区：[1]兰州医学院第二附属医院,730030

出　　处：《肿瘤防治研究》2001年第3期173-175,共3页Cancer Research on Prevention and Treatment

摘　　要：目的　观察端粒酶反义寡聚脱氧核苷酸对人肝癌细胞株的作用。方法　用端粒酶反义寡聚脱氧核苷酸与人肝癌细胞株共同孵育一定时间后 ,观察细胞形态变化 ,观察细胞 DNA含量的分布 ,测定端粒酶活性。结果　端粒酶反义寡聚脱氧核苷酸能诱导人肝癌细胞凋亡 ,抑制端粒酶活性 ,在形态学上表现为细胞膜起泡、染色质固缩、核碎裂、凋亡小体形成 ;电泳呈凋亡特征性 Ladder带 ;流式细胞仪分析显示 ,在 G1期前出现亚 2倍体凋亡峰 ;端粒酶活性抑制。结论　端粒酶反义寡聚脱氧核苷酸能诱导肝癌细胞凋亡 ,抑制端粒酶活性 ,抑制端粒成 ,从而抑制肝癌细胞的生长。Objective To observe the effect of telomerase antisense oligodeoxynucleotides on hepatoma cell line.Methods Hepatoma cell line were cultured with 1~5 μmol/L Telomerase antisense oligodeoxynucleotides for various duration,cell activity,morphologic changes and cell\|DNA distribution were studied.The apoptotic cells were measured with flow cytometer.Telomerase activity were detected by TRAP\|ELISA.Results Telomerase antisense oligodeoxynucleotides acted as a specific growth inhibitor to hepatoma cell line.Hepatoma cells cultured with 5 μmol/L telomerase antisense ollgodeoxynucleotldes presented apoptotic features:i.e. Intace cell membrane ,chromatin condensation,nucleic fragmentation and apoptotic body formation;flow cytometry analysis showed a sub\|G1 cell peak;agarose electrophoresis showed marked DNA ladder.Telomerase activity were inhibited.Conclusion Telonerase may play a crucial role in the growth of hepatoma cells.Telomerase antisense oligodeoxynucleotides may inhibit telomenasre activity.It may be a new target of treatment to hepatoma.

关 键 词：肝癌细胞株 端粒酶活性 反义寡聚脱氧核苷酸 细胞凋亡 实验研究 基因治疗 

分 类 号：R735.7[医药卫生—肿瘤]