ABC免疫印迹技术在分析抗肿瘤McAb识别抗原特性中的应用  

USE OF ABC IMMUNOBLOT IN ANALYSING THE CHARACTER OF ANTIGENS IDENTIFIED BY ANTI-CANCER MCABS

作　　者：王笑利[1] 陈伟[1] 马士英[1] 安天义[1] 刘民培[1] 

机构地区：[1]沈阳军区总医院医学实验科

出　　处：《上海免疫学杂志》1991年第1期35-37,共3页Shanghai Journal of Immunology

摘　　要：利用ABC免疫印迹技术分析一组共16个抗大肠癌McABS识别抗原的特性,效果满意。该法不需分离纯化抗原和制备腹水,使用杂交瘤培养上清液即可得到清晰、准确的识别抗原区带,背景浅淡,用来分析识别抗原分子量及理化性质,特异性强、重复性好、敏感度高。可检测的最小肿瘤抗原蛋白量为1.25×10^(-6)mg,明显优于PAP免疫印迹和间接酶免疫印迹法。本文提示,ABC免疫印迹是分析复杂而微量的抗原的一种有效方法。Characters of 16 antigens idenified by anti-colon cancer McAbs were analysed by immunoblot technique with avidin-biotin peroxidase complex (ABC-Ib). The results showed that ABC-Ib possesses high sensitivity, reproducibility and specificity. Clear and accurate identified antigen bands on light background were gained with simple culture supernatant of hybrid. The minimum amount of protein of tumor tissue antigen detected is 1.25 × 10-6mg. It is suggested that ABC-Ib is very effective on studying complex and trace amount antigen.

关 键 词：免疫印迹法 抗肿瘤McAb 抗原 

分 类 号：R730.3[医药卫生—肿瘤]