GTF-PAc融合防龋DNA疫苗研究(Ⅲ)pGLUA-P免疫定菌鼠防龋研究  

Study of GTF-PAc Fusion Anticaries DNA Vaccine (Ⅲ): Immunization with pGLUA-P Against Dental Caries in Gnotobiotic Rats.

作　　者：贾荣[1] 樊明文[1] 边专[1] 郭继华[1] 陈智[1] 

机构地区：[1]武汉大学口腔医学院,湖北武汉430079

出　　处：《口腔医学纵横》2002年第1期1-4,共4页Journal of Comprehensive Stomatology

基　　金：国家自然基金资助项目 (编号 :39770 799)

摘　　要：目的 :了解GTF -PAc融合防龋DNA疫苗 pGLUA -P激发机体免疫反应和抑制龋病的能力。 方法 :将pGLUA -P和携带 pac基因A -P基因片段的DNA防龋疫苗 pCIA -P以颌下腺周围区域皮下注射 (TSG)和股四头肌注射途径分别免疫定菌SD大鼠 ,以ELISA法检测血清和唾液中的抗体水平 ,采用Keyes法评估大鼠磨牙患龋情况。结果 :pGLUA -P和 pCIA -P经TSG免疫及 pGLUA -P经股四头肌注射免疫组的血清抗PAc的IgG抗体水平明显高于对照组 (P <0 .0 5 ) ,pGLUA -P和pCIA -P经TSG免疫组的唾液抗PAc的IgA抗体水平明显高于其余组 (P <0 .0 5 ) ;pGLUA -P经TSG免疫组的釉质龋和牙本质浅龋记分最低 (P <0 .0 5 ) ;经TSG免疫 pGLUA-P组和pCIA -P组的牙本质中龋记分最低 (P <0 .0 5 )。结论 :GTF -PAc融合防龋DNA疫苗pGLUA -P可以有效地诱导机体的免疫反应 ,抑制龋病的发生和发展 ,防龋效果优于防龋DNA疫苗 pCIA -P。To study the anticaries and inducing immune reaction effects of GTF-PAc fusion anticaries DNA vaccine pGLUA-P in gnotobiotic rats.Methods:Sprague-Dawley rats were immunized with pGLUA-P which carried the GLU fragment of gtfB gene and A-P fragment of pac gene from Streptococcus mutans by targeted salivary gland immunization(TSG)or intramuscular injection(i.m.), or immunized with pCIA-P which carried the A-P fragment of pac gene by TSG; the antibodies in sera and saliva were checked by ELISA; the presence of caries was examined and scored following Keyes'procedure.Results:TSG and i.m. groups immunized with pGLUA-P or pCIA-P displayed significant anti-PAc specific serum IgG antibodies( P <0.05); TSG groups immunized with pGLUA-P or pCIA-P displayed significant anti-PAc saliva IgA antibodies ( P <0.05) and significant lower dental lesions( P <0.05); TSG group immunized with pGLUA-P displayed lowest enamel lesions ( P <0.05). Conclussions:GTF-PAc fusion anticaries DNA vaccine pGLUA-P could effectively induce immune reaction, and could prevent rats from dental caries more effectively than anticaries DNA vaccine pCIA-P. [

关 键 词：龋齿 DNA疫苗 GTF-PAc 疾病预防 变形链球菌 

分 类 号：R780.1[医药卫生—口腔医学]