温热顺铂对肝癌细胞的杀伤效应及其诱导凋亡的研究  

Studies on the effect of cell killing and relationship between heated-CDDP and induction of apoptosis in Hca-F

作　　者：邹丽娟[1] 陈亚敏[2] 徐晓颖[1] 李秀荣[1] 董志[1] 

机构地区：[1]大连医科大学第二临床学院肿瘤科,116027 [2]大连医科大学第一临床学院肿瘤科,116027

出　　处：《肿瘤防治研究》2002年第1期12-14,共3页Cancer Research on Prevention and Treatment

摘　　要：目的 探讨温热 ＣＤＤＰ对 Ｈｃａ－Ｆ细胞的生长抑制、温度与药效的关系以及诱导凋亡的作用。方法 采用ＭＴＴ法，透射电镜，ＤＮＡ电泳以及流式细胞术分析法。结果ＣＤＤＰ对Ｈｃａ－Ｆ细胞有明显的细胞毒性，其ＩＣ５０值为０．５７μｇ／ｍｌ。ＣＤＤＰ合并高温（＞４１℃）加热明显提高了ＣＤＤＰ的杀伤力，二者之间存在着协同增敏作用。其作用强度呈现对药物浓度、作用时间与加热温度的正相关性，且在一定浓度范围内以谤导肿瘤细胞凋亡的发生为主。结论 加热（＞４１℃）顺铂提高了顺铂的细胞毒性。诱导细胞凋亡的发生是其抗肿瘤作用的主要机制之一。Objective To determine the effect of inhibition of cell growth of hyperthermia in combination with CDDP on Hca-F cell lines, and the relationship between temperature and medical effect as well as re- lationship between heated-DDP and induction of apoptosis in Hca-F. Methods Observing the relationship between temperature and effect of CDDP by typanblue assay and transmission electron microscopic tech- nique and flow cytometry as well as DNA fragmentation. Results Heating-CDDP improve the effect of inhi- bition cell growth obviously, temperature and CDDP was interaction each other, and the cell growth inhib- itory effect of heating-CDDP was dose-v,time-and temperature-dependent. induction of apoptosis in Hca-F cell lines was major cell kill way within a limited range. Conclusion Heating can improved the cytotoxicity of CDDP significantly. The induction of apoptosis was ascribed to mechanism of antitumour activity of heating-CDDP.

关 键 词：顺铂 肝癌 细胞毒性 细胞凋亡 化疗 

分 类 号：R739.7[医药卫生—肿瘤]