腹侧海马强啡肽在癫痫敏感性增强形成中的表达  

A Expression of Dynorphin A(1-8) in Ventral Hippocampus during Induction of Enhanced Seizure Susceptibility

作　　者：王静[1] 李艺影[1] 李小勇[1] 张万琴[2] 

机构地区：[1]北京市神经外科研究所 [2]大连医科大学生理教研室

出　　处：《首都医科大学学报》2001年第4期352-354,共3页Journal of Capital Medical University

摘　　要：用SD大鼠建立红藻氨酸 (KA)诱发实验性颞叶癫痫动物模型 ,利用免疫细胞化学方法 (ICC) ,研究KA诱发的癫痫敏感性增强形成过程中 (KA后 1～ 7d) ,大鼠腹侧海马内强啡肽 [DYNA(1 8) ]免疫反应活性变化的时间过程。发现 :KA后 2d ,大鼠腹侧海马齿状回颗粒细胞 (DGC)层出现强啡肽免疫反应阳性神经细胞 ,KA后 4d ,其阳性神经细胞数量及免疫反应强度达高峰 ,KA后 5～ 7d,免疫反应逐渐消失。研究表明 :腹侧海马DGC层强啡肽免疫反应阳性神经细胞消失出现的时间过程 (KA后 5～ 7d)与KA诱发的癫痫敏感性增强形成出现的时间过程 (KA后 5～ 7d)一致 。To study a role of dynorphin A(1 8)(DYN A(1 8)) in ventral hippocampus during induction of enhanced seizure susceptibility, an acute model as human temporal lobe epilepsy was made by kainic acid (KA) causing seizure episode in the rats. The time course of alteration of dynorphin A(1 8) like immunoreactivity in ventral hippocampus during induction of enhanced seizure susceptibility ( 1～7 d after KA ) was detected by immunocytochamical method. SD rats were given 10 mg/kg,S.C. of KA systemically. Two days later, dynorphin A(1 8) immunoreactive neurons appeared in the nuclei of dentate granule cells (DGC) in ventral hippocampus. The maximal intensity of staining and the largest number of the immunoreactive dynorphin A(1 8) occurred 4 d after KA. Five to seven days after KA, during induction of enhanced seizure susceptibility, dynorphin A(1 8) immunoreactive neurons also gradually disappeared in DGC of ventral hippocampus. Endogenous dynorphin in the seizure sensitive ventral hippocampus appeares to play an important role in regulation of brain excitability, especially in the induction of enhanced seizure susceptibility.

关 键 词：癫痫 敏感性 腹侧海马 红藻氨酸 强啡肽 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R338.26[医药卫生—临床医学]