VEGF反义寡核苷酸对C_6胶质瘤细胞VEGF表达抑制作用的定量和定性检测  

INHIBITION OF VEGF EXPRESSION IN C_6 GLIOMA CELLS WITH VEGF ANTISENSE OLIGONUCLEOTIDES

作　　者：李维方[1] 张光霁[1] 朱诚[1] 金由辛[2] 卢亦成[1] 

机构地区：[1]第四军医大学长征医院 [2]中国科学院生物化学研究所

出　　处：《解放军医学杂志》2002年第1期32-33,I002,共3页Medical Journal of Chinese People's Liberation Army

基　　金：上海市优秀学科带头人资助计划基金资助课题 (编号 99XD14 0 0 2 )

摘　　要：为观察VEGF反义寡核苷酸对C6胶质瘤细胞VEGF表达抑制作用 ,应用免疫荧光流式细胞技术和免疫细胞化学定量和定性观察反义、正义、错义寡核苷酸对C6胶质瘤细胞VEGF表达的影响。结果显示 ,VEGF反义寡核苷酸对C6胶质瘤细胞VEGF的表达有明显抑制作用 ,而且抑制作用存在浓度依赖性。提示VEGF反义寡核苷酸能够有效地抑制C6胶质瘤细胞VEGF的表达。To observe the inhibition of vascular endothelial growth factor(VEGF) expression in C 6 glioma cells with VEGF antisense oligonucleotides.VEGF expression in C 6 glioma cells was examined quantitatively and qualitatively with immunofluorescence flow cytometric analysis and immunohistochemistry.Results showed that VEGF antisense ODNs inhibited the VEGF expression in C 6 glioma cells,and the inhibition was concentration dependent.This indicates that VEGF antisense ODNs can effectively suppress the VEGF expression in C 6 glioma cells.

关 键 词：内皮生长因子 反义寡核苷酸类 神经胶质瘤 

分 类 号：R730.264[医药卫生—肿瘤]