1,25(OH)_2D_3对原代白血病细胞的诱导分化作用  

INDUCTION OF DIFFERENTIATION OF FRESH HUMAN MYELOID LEUKEMIA CELLS BY 1,25-DIHYDROXYVITAMIIN D_3

作　　者：程涛[1] 严舫[1] 陈方道 

机构地区：[1]第二军医大学附属长海医院血液科

出　　处：《上海医学》1991年第11期635-637,共3页Shanghai Medical Journal

摘　　要：观察22例髓性白血病的骨髓细胞在体外原代培养中对1,25(OH)_2D_3(简称 D_3)的反应,发现 D_3能使 NBT 阳性率和成熟细胞百分率增加(P<0.05),说明 D_3对白血病细胞有促分化作用。通过测定10例初发 AML 细胞 NBT 阳性细胞数和 CFU-AML 产率,进一步证实了 D_3对 AML 细胞的诱导分化作用(P<0.05)和增殖抑制作用(P<0.01)。但是,上述诱导分化作用并不完全,从而解释临床应用不理想的原因。个体化分析后发现诱导分化并非 D_3发挥治疗作用的唯一途径。Bone marnow cells from 22 patients with myeloid leukemia incubated in vitro with1,25-dihydroxyviatmin D_3(D_3),and the induction of differentiation were studied.D_3 couldinduce the leukemic cells to differentiate into mature cells shown by the increase of NBT+(P<0.05).In 10 patients with acute myeloid leukemia(AML),the differentiation andclonal growth inhibition confirmed by the absolute NBT+cell number(P<0.05)and theCFU-AML(P<0.01)respectively were also observed.But the effect of differentiationwas not complete,and the unsatisfactory clinic result of D_3 could be thus explained.Thebetter ways for clinic use of D_3 are discussed.

关 键 词：白血病 D3 诱导分化剂 

分 类 号：R733.705[医药卫生—肿瘤]