细胞学联合端粒酶检测对贲门癌诊断价值的探讨  

Detection of telomerase activity and cytology in the diagnosis of cardiac cancer.

作　　者：王强[1] 吴清明[1] 李胜保[1] 

机构地区：[1]郧阳医学院附属十堰市太和医院消化内科,湖北十堰442000

出　　处：《中国实用内科杂志》2001年第7期400-401,共2页Chinese Journal of Practical Internal Medicine

摘　　要：目的　探讨胃镜直视下刷取贲门粘膜脱落细胞中端粒酶活性及细胞学对贲门癌的诊断价值。方法　采用TRAP PCR银染定性法及TRAP PCR ELISA定量法对 72例贲门粘膜刷落细胞进行端粒酶活性分析 ,同时进行细胞学诊断及病理学诊断。结果　贲门癌组端粒酶活性 (1 5 2 1± 0 192 ,OD值 )明显高于贲门炎组 (0 0 65±0 0 14,OD值 ) ,端粒酶在贲门癌组阳性率 (88 89% )明显高于贲门炎组 (11 11% )。端粒酶在贲门癌组的阳性率明显高于贲门粘膜脱落细胞学的阳性率 (77 78% )。端粒酶联合细胞学诊断可使贲门癌的诊断率提高到93 33%。结论　贲门粘膜脱落细胞学诊断联合端粒酶活性测定可作为诊断贲门癌特异而敏感的方法 。Objective To investigate the diagnostic significance of cytology and telomerase activity in the exfoliated cells of cardia obtained from endoscopic brushing in the cardiac cancer.Methods The technique of the qualitative TRAP siver staining and quantitative TRAP PCR ELISA were employed to detect telomerase activity in the exfoliated cells of cardiac obtained from endoscopic brushing in 72 cases with cardial lesions,cytological diagnosis was made at the same time.Results Telomerase activity with cardiac cancer group(1\^521±0\^192)was significantly higher than that with cardialitis group(0\^065±0\^014).Positive rate of telomerase activity detected in cardiac cancer group(88\^89%)was significantly higher than that with cardialitis group(11\^11%).Positive rate of telomerase activity detected in cardiac cancer group(88\^89%)was significantly higer than cytological examination(77\^78%).The diagnostic rate of cardiac cancer was improved to 93\^33% if telomerase activity and cytology were examined at the same time.Conclusion Results show that the combination of cytology and telomerase activity in the exfoliated cardiac cells may be an effective and sensitive method in the diagnosis of cardiac cancer.This research can be a basis for the mass screening of cardiac cancer.

关 键 词：贲门肿瘤 端粒酶 细胞学 刷落细胞 

分 类 号：R735.2[医药卫生—肿瘤]