p16蛋白在喉癌前期病变中的表达及意义  

Expression of p16 protein in the precancerous condition of laryngeal carcinoma

作　　者：吴昌竹[1] 李惠萍[2] 宋伟[1] 于博[1] 

机构地区：[1]解放军202医院耳鼻咽喉-头颈外科,辽宁沈阳110003 [2]中国医科大学第一临床医院耳鼻咽喉科,辽宁沈阳110006

出　　处：《中国耳鼻咽喉颅底外科杂志》2001年第4期242-244,I008,共4页Chinese Journal of Otorhinolaryngology-skull Base Surgery

摘　　要：目的　通过喉粘膜上皮中 p16表达的实验 ,讨论p16蛋白在喉癌发生过程中的作用。 方法　用S -P法检测 10 6例喉癌前期病变标本中 p16蛋白的表达 ,并设 30例喉正常上皮和 32例喉鳞癌作对照。 结果　p16蛋白在喉正常粘膜上皮中阳性率为86 .6 7% ;喉癌前期病变中为 75 .47% ,喉鳞癌中为 37.5 % ,两者差异有显著性 (P <0 .0 5 )。喉癌前期病变随病变程度加重 p16阳性率呈下降趋势 ,单纯增生组 (83.33% )与重度不典型增生组 (5 5 .5 6 % )差异有非常显著性 (P <0 .0 1)。结论　p16基因表达的缺失与喉癌的发生有关 ;p16蛋白的表达可作为判断喉癌前期病变向恶性肿瘤转化危险性的一个参考指标。Objective To determine the role of p16 protein in the occurrence of laryngeal cancer by testing the expression of p16 protein in the mucosa epithelium of larynx. Methods p16 samples of precancerous condition were tested by S-P method. In the meanwhile, 30 normal laryngeal epithelium and 32 laryngeal squamous cancers were studied as controls. Results Positive rate of p16 protein was 86.67% in normal mucosa epithelium, 75.47% in precanerous condition and 37.5% in laryngeal squamous cancer. There was distinctive difference between precancerous condition and laryngeal squamous cancer (P< 0.05). Expression of p16 descended with the precancerous condition strengthening. Expression of p16 in group of pure hyperplasia was considerably different from expression in group of serious non-typical hyperplasia (P< 0.01). Conclusion Shortage of p16 expression is related to the occurrence of laryngeal cancer. Expression of p16 protein can be regarded as an index to judge whether precancerous condition worsens.

关 键 词：喉肿瘤 病理学 癌前状态 P16蛋白 鳞状细胞癌 

分 类 号：R739.65[医药卫生—肿瘤]