斑马鱼胚胎发育技术在毒性评价中的应用  

Utilization of embryo development technique of Brachydanio rerio to evaluateing toxicity on various chemicals.

作　　者：朱琳[1] 史淑洁[1] 

机构地区：[1]南开大学环境科学与工程学院,天津300071

出　　处：《应用生态学报》2002年第2期252-254,共3页Chinese Journal of Applied Ecology

基　　金：高等学校重点实验室访问学者基金;南京大学污染控制与资源化研究国家重点实验室基金资助项目

摘　　要：对斑马鱼 (Brachydaniorerio)胚胎发育技术在环境科学领域的应用作一综述 .斑马鱼胚胎毒性技术是各国际标准组织认可的标准毒性测定方法之一 ,属于致畸效应检验 .该项技术成本低、易操作、灵敏度高 ,特别是具有可记录多项毒性指标的特点 ,并可以此判断污染物的致毒机理 .斑马鱼胚胎发育过程受重金属影响较大 ,其中Cu的毒性最强 ,Hg次之 ,Cr最弱 ;有机农药中三苯基锡类 (TPTA)的毒性最强 ,林丹次之 ;有机试剂中含卤素取代基和苯胺类毒性最大 .这与其它毒性测定方法的结果完全一致 ,并表现出较高的灵敏度 ,特别是选用非致死性的指标 .可以初步认定这对测定复合污染物毒性并分析毒物的致畸效应方面有很好的发展前景 .The application of Brachydanio rerio embryo development technique to research on environmental science was summarized in this paper.This testing technique was approved as one of standard methods for chemical toxicity test,especially for teratogensis,by international organizations (OECD,DIN).There are many advantages of this testing, e.g.,low cost, easy operation,high sensitivity and multiple sensible endpoints,comparing with other toxicity testing.Also,it was used to judge toxic mechanism of chemicals.The embryo develompent of zebrafish can be affected obviously by heavy metal,pesticide,organic reagent and complex chemicals.Among them,Cu and Hg appeared stronger toxicity,and Cr was weaker for heavy metals.TPTA and Lindane were higher toxicity within pesticide,and they were higher toxicity if the organic reagent with one or more halogen substituent (s) and aminobenzene.These results were correspondent with other toxicity testing,and it showed higher sensitivity,especially a sublethal endpoint was selected.Therefore,it can be expected to test toxicity and teratogensis on mixed pollutant monitoring.

关 键 词：斑马鱼 胚胎发育 毒性评价 应用 污水 

分 类 号：X174[环境科学与工程—环境科学] X503.225