人胎儿晶状体上皮细胞增殖及相关因子的动态研究  

Dynamic study on the proliferation and relevant factors in lens epithelial cells of human fetus

作　　者：牛嗣云[1] 高福禄[1] 庞小静[1] 李秀华[2] 张玉娟[2] 王瑞学[3] 

机构地区：[1]河北省承德医学院组织胚胎学教研室,067000 [2]承德医学院附属医院 [3]河北省承德卫生学校

出　　处：《眼科研究》2002年第1期35-38,共4页Chinese Ophthalmic Research

基　　金：河北省教育厅资助(2000111)

摘　　要：目的观察增殖细胞核抗原（ＰＣＮＡ）及转化生长因子－β１（ＴＧＦ－β１）、表皮生长因子（ＥＧＦ）在人胎儿晶状体上皮细胞的表达，并探讨其在晶状体发育中的作用。方法用ＳＰ免疫细胞化学法对４８例人胎儿不同胎龄晶状体上皮细胞进行ＰＣＮＡ，ＴＧＦ－β１，ＥＧＦ免疫细胞化学染色。结果ＰＣＮＡ，ＴＧＦ－β１，ＥＧＦ在人胎儿不同胎龄晶状体上皮细胞内均有表达；免疫阳性细胞随着胎龄的增加而减少。ＰＣＮＡ阳性细胞核为桔黄色或棕黄色；ＴＧＦ－β１，ＥＧＦ阳性细胞浆为桔黄色或棕黄色的颗粒状或斑块状；经相关分析，ＴＧＦ－β１与ＥＧＦ以及ＴＧＦ－β１，ＥＧＦ与ＰＣＮＡ均呈正相关。结论ＰＣＮＡ，ＴＧＦ－β１，ＥＧＦ在人胎儿不同胎龄晶状体上皮细胞内均有表达；ＴＧＦ－β１，ＥＧＦ具有协同作用，共同促进晶状体上皮细胞的增殖。ObjectiveTo observe the expressions of proliferating cell nuclear antigen (PCNA),transforming growth factor-β1 (TGF-β1)and epidermal growth factor (EGF) in lens epithelial cells (LECs) of human fetus and study their roles in the lens development.MethodsSP immunocytochemical techniques were applied for PCNA,TGF-β1 and EGF in LECs of different fetal ages.ResultsThe expressions of PCNA,TGF-β1 and EGF were found in LECs of different fetal ages.The numbers of positive cells were decreased with the growth of the fetus.The positive staining for PCNA was orange or brown in the nucleus.the positive staining for TGF-β1 and EGF were orange or brown and were scattered or mottled within the cytoplasm.the positive correlation was found between TGF-β1 and EGF,TGF-β1 and PCNA,EGF and PCNA.ConclusionThere are all expressions of PCNA,TGF-β1 and EGF in LECs of different fetal ages.TGF-β1 and EGF have associated action in promoting the proliferation of LECs.

关 键 词：晶状体上皮细胞 增殖细胞核抗原 转化生长因子-Β1 表皮生长因子 人胎儿 细胞增殖 动态研究 

分 类 号：R770.2[医药卫生—眼科]