植物组织培养中消毒剂的运用  

A study on the use of disinfectants in plant tissue culture

作　　者：王利民[1] 周毅[1] 陈龙友[1] 张宇斌[1] 

机构地区：[1]贵州师范大学生物科学技术系,贵州贵阳550001

出　　处：《贵州师范大学学报（自然科学版）》2002年第1期15-17,共3页Journal of Guizhou Normal University：Natural Sciences

摘　　要：对月季 (Roeschinensis)、香石竹 (Dianthuecaryophllus)、鱼腥草 (Houttuyniacordata)组织培养中几种常用消毒剂Ca(ClO) 2 、HgCl2 、CH3 CH2 OH、H2 O2 、漂白粉 (饱和溶液 )的运用进行了比较研究 ,结果表明 :0 1%HgCl2 消毒效果最好 ,污染率较低 ,但成活率也较低 ;用地下茎作材料进行组织培养时 ,75 %CH3 CH2 OH配合 0 1%HgCl2This article compares the use of different disinfectants, Ca(CIO) 2,HgCI 2,H 2O 2, alcohol and saturate bleaching powder solution in tissue culture of Roes chinensis,Dianthue caryophllus and Houttuynia cordata. It has showed the disinfection effect of 0.1?% HgCl 2 is the best as the plan pollution rate is low. However, the survival rate is also low. When the underground stems are taken as tissue culture's material,it is more effective to use the 0.1% HgCl 2 with 10?% alcohol.

关 键 词：植物组织培养 消毒剂 污染率 成活率 次氯酸钙 氯化汞 双氧水 

分 类 号：Q943.1[生物学—植物学]