肺癌术后转移及预后与转移抑制基因nm23和nm23-H_l的关系  

Relationship between nm23,nm23-H_1 and postoperative invasion,metastasis and prognosis of non-small cell lung cancer

作　　者：陈晓峰[1] 丁嘉安[1] 王海峰[1] 何国钧[2] 周清华[3] 张尚福[3] 

机构地区：[1]上海市肺科医院胸外科 [2]上海市肺科医院呼吸科 [3]华西医科大学附属第一医院胸外科

出　　处：《中华实验外科杂志》2002年第1期40-41,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目 (39470 687) ;上海市卫生局科技发展基金资助项目 (9942 4 )

摘　　要：目的　探讨人非小细胞肺癌中nm2 3、nm 2 3 H1的表达水平与肺癌的浸润、转移和预后的关系。方法　应用免疫组织化学法对 14 6例人非小细胞肺癌中nm 2 3、nm 2 3 H1的表达水平进行研究 ,用Cox比例风险模型进行多因素分析。结果　肺癌中nm 2 3、nm2 3 H1表达水平 (71.2 3±5 .19) % ,(64 .98± 5 .72 ) %均明显低于癌旁肺组织 (89.0 0± 7.2 1) %和正常肺组织 (90 .66±6.78) % (P <0 .0 5 ) ,肺癌中nm2 3、nm 2 3 H1的表达水平与肺癌的细胞分化程度、是否存在转移有密切关系 (P <0 .0 5 ) ,nm 2 3 H1高表达组术后 3年及 5年 (78.81%及 5 5 .73 % )生存率均明显高于低表达组 (2 1.42 %及 17.83 % ) (P <0 .0 5 )。Cox比例风险模型分析显示影响肺癌患者术后预后的前 4种因素依次为nm 2 3 H1表达水平 ,TNM分期 ,淋巴结受侵状况和原发肿瘤大小。结论　nm2 3、nm 2 3 H1在肺癌中起转移抑制基因的作用 ,它们的表达水平降低可能是肺癌转移的重要原因之一。Objective To explore the expression level of nm23,nm23 H1 gene and its relationship to invasion,metastasis and prognosis in human non small cell lung cancer (NSCLC).Methods The expression level of nm23,nm23 H 1 gene product was detected by immunohistochemistry in 146 non small cell lung cancer (NSCLC) samples.Coxs multivariate analysis was performed.Results The positive rate of nm23,nm23 H 1 expression in NSCLC [(71.25±5.19)%,(64.98±5.72)% respectively] was significantly lower than in the adjacent non tumor lung tissues (89.00±7.21)% and that in normal lung tissues (90.66±6.78)%(P<0.05).There were significant differences between nm23,nm23 H 1 expression and the degree of differentiation of the tumor and tumor metastasis (P<0.05).Three year and 5 year survival rate of patients with high nm23 H 1 expression (78.81%,55.73% respectively) was significantly higher than those with low nm23 H 1 expression.The Cox proportional hazard regression model revealed 4 important factors pertaining to patient survival rate as following: nm23 H 1 expression level,TNM stages,lymph node metastasis and size of primary tumor.Conclusion nm23 gene and nm23 H 1 gene suppressed tumor metastasis in NSCLC and the decrease in their expression may be one of the essential causes of lung cancer metastasis.

关 键 词：肺肿瘤 肿瘤转移 预后 肿瘤抑制基因 免疫组织化学方法 

分 类 号：R734.2[医药卫生—肿瘤]