喜树碱、胺苯吖啶、长春花碱对淋巴细胞性和髓样白血病细胞周期的影响  

Effects of Camptothecin,Amsacrine and Vinblastine on the cell cycle of lymphocytic or myelogenous leukemia cells.

作　　者：陈义发[1] 龚建平[1] 吴在德[1] 裘法祖[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院普外科,武汉430030

出　　处：《中华实验外科杂志》2002年第1期44-45,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目 (39670 365 ;39730 2 70 ) ;国家杰出青年科学基金资助项目 (3972 50 2 7) ;卫生部临床学科重点项目(2 0 2 0 1 0 6)

摘　　要：目的　比较 3种不同的抗肿瘤药物 (喜树碱、胺苯吖啶、长春花碱 )对培养的淋巴细胞性 (Molt 4)或髓样白血病细胞 (HL 60 )的细胞周期的影响。方法　在体外用喜树碱、胺苯吖啶或长春花碱处理诱导HL 60或Molt 4细胞 3、6、9h后收获 ,75 %的冷乙醇固定 2 4h以上。用溴化丙啶对细胞核DNA染色 ,FACSort流式细胞仪分析细胞周期并依据Sub G1法计算G1、S、G2 /M期细胞或凋亡细胞的百分率。结果　喜树碱能选择性地诱导HL 60细胞的S和G2 /M期细胞凋亡 ,总凋亡率可达 41.2 7% ;胺苯吖啶仅能使部分Molt 4细胞阻滞于G2 /M期 ,阻滞率为 9.2 4% ;长春花碱使Molt 4细胞阻滞于G2 /M期阻滞率为 11.3 0 %。结论　HL 60细胞对喜树碱的细胞毒作用异常的敏感 ,易于发生凋亡 ;胺苯吖啶诱导Molt 4细胞阻滞于G2 /M期 ;长春花碱诱导Molt 4细胞阻滞于有丝分裂前期。Objective To compare the diverse effects of three kinds of antitumor drugs [Camptothecin (CAM),Amsacrine (AMSA),Vinblastine (VIN)] on the cell cycle of lymphocytic cell line (Molt 4) or human promyelocytic cell line (HL 60).Methods HL 60 or Molt 4 cells were induced by CAM, AMSA or VIN in vitro for 3,6,9?h,then the cells were fixed with 75% cold ethanol over 24?h followed by cell staining with propidium iodide as the DNA fluorochrones.FACSort flow cytometry was used to analyze the percentage of G 1,S,G 2/M or apoptosis(Sub G 1)of the cells in cell cycle.Results CAM selectively triggered apoptosis of S and G 2 M phase of HL 60 cells,with the apoptosis rate being 41.27%.AMSA could partly block the Molt 4 cells at G 2/M phase block with the arrest rate beig 9.24%. VIN resulted in Molt 4 cells to be arrested at G 2/M phase significantly with the arrest rate being 11.30%.Conclusion HL 60 cells may be especially sensitive to the cytotoxic action of CAM and prone to apoptosis; AMSA induced Molt 4 cells to to arrested at G 2/M phase; VIN result in Molt 4 cells to be arrested in mitosis.

关 键 词：喜树碱 胺苯吖啶 长春花碱 细胞周期 白血病 抗肿瘤药物 

分 类 号：R73-3[医药卫生—肿瘤]