安普霉素生物合成途径的研究  

Studies on the biosynthetic pathway of apramycin

作　　者：李相丰[1] 何建勇[1] 田威[1] 韩果红[1] 白秀峰[1] 

机构地区：[1]沈阳药科大学,沈阳110016

出　　处：《中国抗生素杂志》2002年第2期97-100,共4页Chinese Journal of Antibiotics

摘　　要：安普霉素产生菌黑暗链霉菌 (S.tenebrarius) 1- 16经亚硝酸胍 (NTG)、硫酸二乙酯 (DES)诱变筛选获得了生物合成阻断变株。微生物转化实验结果表明 2 -脱氧链霉胺 (2 - DOS)、巴龙霉胺和安普霉胺是安普霉素生物合成的中间体。阻断变株共合成实验结果明确了各阻断变株阻断位点间的关系及上述中间体在安普霉素生物合成中的衍生次序。依据以上实验结果 ,我们提出了安普霉素可能的生物合成途径。Fully blocked mutants of S.tenebrarius 1~16 was obtained by using NTG and DES treatment. Biotransformation experiment results suggested that 2 deoxystreptamine, paromamine and aprosamine were the intermediates of apramycin biosynthesis. Cosynthesis results of blocked mutants elucidated the relationship of the blocked sites of the mutants and the relative position of above compounds in the biosynthetic pathway of apramycin. A biosynthetic pathway of apramycin was proposed with the above results.

关 键 词：安普霉素 阻断变株 生物合成途径 抗生素 微生物转化 

分 类 号：R978.12[医药卫生—药品]