用壳聚糖作为配基载体亲和层析提纯淀粉糖化酶的研究  

Affinity Chromatograpy Using Chitosan as Ligand Carrier for the Purification of Glucoamlyase

作　　者：陈天[1] 隋德新[1] 姜涌明[1] 叶秀莲[1] 

机构地区：[1]江苏农学院生化教研室,扬州225001

出　　处：《生物化学杂志》1991年第4期421-424,共4页

摘　　要：以自制的壳聚糖作配基载体,植物血球凝集素(PHA)作配基,通过戊二醛交联研制成一种用于淀粉糖化酶提纯的新型亲和吸附剂。对淀粉糖化酶的亲和层析研究表明:提纯倍数为1.8,酶活性收率达80％,纯度经聚丙烯酰胺凝胶等电聚焦电泳(IEF-PAGE)鉴定为一条带,没有非特异性吸附作用。具有简单、安全、快速和高收率等优点。A novel affinity chromatography technique is constructed which employs chitosan-PHA affinity absorbent to purify glucoamlyase. This process is demonstrated with chitosan as ligand carrier and PHA as the affinity ligand. PHA is convalently bound to chitosan by glutaraldehyde. The proportion of binding PHA to chitosan is determined by indirected method. The results of affinity chromatography for gluco-amlyase show that recovery of glucoamlyase activity reaches 80%, purity examined by IEF-PAGE is shown by presence of a single band. The affinity chromatography column can be used repeatedly, purification efficiency is not decreased, without any non-specific binding. This procedure presents few advantages, such as safety, simplicity, quickness, high recovery and so on.

关 键 词：淀粉糖化酶 亲和层析 壳聚糖 

分 类 号：Q550.3[生物学—生物化学]