上皮性卵巢癌中PTEN突变分析  

Analysis of PTEN mutation in epithelial ovarian cancer.

作　　者：车艳辞[1] 姚勤[1] 戴淑真[1] 罗兵[2] 

机构地区：[1]青岛大学医学院附属医院妇产科,青岛266003 [2]青岛大学医学院微生物学教研室

出　　处：《现代妇产科进展》2002年第1期18-20,共3页Progress in Obstetrics and Gynecology

摘　　要：目的 :探讨抑癌基因PTEN与上皮性卵巢癌的发生发展之间的关系。方法 :收集上皮性卵巢癌 6 0例 (浆液性 4 7例 ,粘液性 13例 )和良性上皮性卵巢肿瘤 10例及正常卵巢组织 5例 ,提取组织DNA后用PCR SSCP方法检测PTEN基因的第 5、8外显子的突变。结果 :3例 (2例浆液性腺癌、1例粘液性腺癌 )突变 ,并经DNA测序结果证实。结论 :在上皮性卵巢癌的发生发展过程中PTEN突变可能起作用 ,但不能除外该基因有突变以外的其他异常改变参与了肿瘤的发生。Objective:To explore the relationship between the tumor suppressor gene PTEN and tumorigenesis of epithelial ovarian cancer.Methods:Sixty epithelial ovarian cancers(including 47 serous,13 mucinous)and 10 benign epithelial ovarian tumors as well as 5 normal ovarian tissues for mutations in exon 5,8 of PTEN were analyzed by PCR SSCP.Results: Three cases of PTEN mutations were found in all of the tumors and normaltissues,one was mucinous cancer and the other two were serous cancer. Conclusions:Somatic PTEN mutations might play a subordinate role in the tumorigenesis of epithelial ovarian cancers,but some changes other than mutation of PTEN might be related to it.

关 键 词：卵巢肿瘤 聚合酶链反应 DNA序列分析 PTEN突变 抑癌基因 

分 类 号：R737.31[医药卫生—肿瘤]