HL-60细胞分化与凋亡关系的初步研究  

STUDY ON THE RELATIONSHIP BETWEEN DIFFERENTIATION AND APOPTOSIS OF HL-60 CELLS

作　　者：何维敬[1] 毕正[1] 马现君[1] 张莲英[2] 李登欣[3] 张建业[2] 

机构地区：[1]山东大学齐鲁医院检验科 [2]山东大学医学院生物化学教研室 [3]潍坊市人民医院内科

出　　处：《山东医科大学学报》2001年第5期420-422,共3页Acta Academiae Medicinae Shandong

基　　金：山东省科委科研基金资助课题 ( 96 0 0 79)

摘　　要：目的 :探讨HL 6 0细胞分化与凋亡的初步关系 ,指导白血病的联合化疗。方法 :NBT还原试验检测细胞分化、流式细胞术 (FCM )检测细胞凋亡及bcl 2、c myc蛋白的表达 ,观察分别经ATRA、TPA诱导分化的HL 6 0细胞对VP 16的凋亡反应。结果 :与对照组相比 ,经ATRA、TPA分化的HL 6 0细胞bcl 2、c myc蛋白表达都明显降低 (P <0 .0 5 ) ,其中以TPA下调c myc蛋白的幅度最大 (76 % ) ;对VP 16的诱导反应 ,经TPA分化的HL 6 0细胞凋亡率明显减少 (P <0 .0 5 ) ,而经ATRA分化的细胞凋亡率则无明显变化 (P>0 .0 5 )。结论 :HL 6 0细胞对VP 16的凋亡诱导反应与bcl 2、c myc蛋白的下调表达程度有关 ;分化诱导剂ATRA与低剂量的VP 16联合应用可望改善AML的化疗效果 ,并且以后者先用效果更好。Objective:Explore the relationship between differentiation and apoptosis of HL-60 cells,to direct the chemiotherapy of acute leukemia.Methods:Use NBT test to detect cell differentiation,flow cytometic (FCM)to analysis apoptotic cells and the expression of bcl-2 and c-myc protein.So study the apoptosis response induced by etoposide (VP-16)in adherent ATRA and TPA-differentiated HL-60 cells and its probably mechanism.Results:The gene expression of bcl-2 and c-myc was significantly lower in ATRA and TPA-differentiated HL-60 cells than that in control( P<0.05 ),c-myc protein levels down-regulated by TPA was the lowest(76%).Compared with control HL-60 cells,the percent of apoptotic cells induced by VP-16 in adherent TPA-differentiated HL-60 cells was apparently decreased ( P<0.05 ),but such changes can't be found in adderent ATRA-differentiated HL-60 cells ( P>0.05 ).Conclusion:The apoptotic respone induced by VP-16 is different in differentiated HL-60 cells and this perhaps due to the down-regulated levels of bcl-2 and c-myc protein.Differentiated drugs such as ATRA combined with low dose of VP-16 can improve the chemotherapy efficiency of AML,while the latter administrated first is better probably.

关 键 词：HL-60细胞 细胞分化 细胞凋亡 白血病 药物疗法 联合化疗 

分 类 号：R733.7[医药卫生—肿瘤]