不同LET碳离子对哺乳动物细胞的生物学效应  

Biological effects on mammalian cells of carbon ions with different LET

作　　者：王菊芳[1] 周光明[1] 李文建[1] 李强[1] 党秉荣[1] 何静[1] 卫增泉 高清祥[2] 陈宏波[2] 

机构地区：[1]中国科学院近代物理研究所,兰州73000 [2]兰州大学生物系

出　　处：《中华放射医学与防护杂志》2001年第5期325-327,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：中科院西部之光资助项目 (XB980 6 0 4)

摘　　要：目的　研究不同传能线密度 (LET)的重离子对体外培养细胞存活率、微核率和染色体畸变的效应。方法　用集落形成法研究细胞的存活情况 ,观察统计细胞微核率和染色体结构的变化。结果　①细胞存活 :不同LET碳离子引起细胞失活效应由大到小的顺序依次为 12 5 ,2 0 0 ,70 0keV μm。②细胞微核 :相同剂量下 ,2 0 0keV μm碳离子所致微核率高于 70 0keV μm时的结果。③染色体畸变 :可看到多种染色体结构变异。结论　在本实验中 ,LET为 12 5keV μm的碳离子引起的失活效应最大 ,2 0 0keV μm碳离子引起的微核率高于 70 0keVObjective\ The effects of cells carbonions with different linear energy transfer(LET) on survival fraction,micronucleus frequency and chromosomal aberrations of cultured cells were investigated. Methods\ The survival fraction was studied by colong\|formation assay and the mironucleus frequency and structural aberration of chromosomes were observed and counted. Results\ ①Survival fraction:The LET order of carbon ions from high to low inducing celluar inactivation was 125,200 and 700 keV/μm.②Micronucleus frequency:Under the same dose,the micronucleus frequency induced by 200 keV/μm carbon ions was higher than that by 700 keV/μm ones.③Chromosomal aberration:A lot of structural aberrations could be observed. Conclusion\ In this study,the maximum celluar inactivation was obtained when LET of carbon ions was 125 keV/μm,the micronucleus frequency caused by carbon ions of 200 keV/μm was higher than that caused by 700 keV/μm.\

关 键 词：传能线密度 碳离子 哺乳动物细胞 存活率 微核率 染色体畸变 体外培养 细胞培养 

分 类 号：R14[医药卫生—公共卫生与预防医学]