银微盘电极上谷胱甘肽降解产物的伏安行为  

Voltammetric Behavior of Degradation Product of Glutathione at Silver Microdisk Electrode

作　　者：李茂国[1] 许发功[1] 方宾[1] 

机构地区：[1]安徽师范大学化学与材料科学学院,安徽芜湖241000

出　　处：《分析测试学报》2002年第1期55-57,共3页Journal of Instrumental Analysis

基　　金：安徽师范大学专项基金资助项目 (125299)

摘　　要：谷胱甘肽是一个含硫三肽 ,基于谷胱甘肽降解产物中的L_半胱氨酸 (RS- )能与Ag+形成难溶化合物的性质 ,在银微盘电极上研究了其水解产物的伏安行为 ;运用示差脉冲溶出伏安法 (DPSV) ,在0.1mol/LHAc -NaAc介质中 ,谷胱甘肽降解产物的还原峰电流与谷胱甘肽浓度于9.0×10-7～4.0×10-5 mol/L范围内有较好的线性关系 ,测定限为5.0×10 -8mol/L ,用于模拟样品测定 。The voltammetric behavior of the degradation product of glutathione , L _ cysteine, at self _ assembled silver microdisk electrode in 0.1 mol/L HAc-NaAc buffer solution was investigated. The mechanism of electrode reaction was also discussed. Based on the production of a hardly soluble matter of L _ cysteine with Ag+, a method for the determination of glutathione was proposed by differential pulse cathodic stripping voltammetry. A linear range of 9.0×10-7～4.0×10-5 mol/L and a limit of determination of 5.0×10-8 mol/L for glutathione were obtained. The method was used for determing glutathione content in artificially mixed samples with satisfactory results.

关 键 词：银微盘电极 谷胱甘肽 降解产物 阴极溶出伏安法 L-半胱氨酸 测定 

分 类 号：Q516[生物学—生物化学] O629.72[理学—有机化学]