黄瓜苗期猝倒病拮抗菌的鉴定  

Identification of bacteria antagonistic to cucumber seedling damping-off.

作　　者：楼兵干[1] 张炳欣[1] Maarten Ryder Stephen Barnett 

机构地区：[1]浙江大学植物保护系,浙江杭州310029 [2]CSIRO Land and Water [3]CSIRO Landand Water

出　　处：《浙江大学学报（农业与生命科学版）》2002年第1期54-58,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：澳大利亚国际农业发展中心资助项目 ( PN9680 );国家自然科学基金资助项目 ( 3 0 0 70 5 11)

摘　　要：通过甲基脂肪酸气相色谱分析法 (GC- FAME)、Biolog细菌鉴定法 (借助于计算机的数值分类法 )和其它试验对黄瓜苗期猝倒病具有明显防治效果的 16株拮抗菌株进行分类鉴定 .结果表明 :黄瓜苗期猝倒病拮抗菌的优势种为 Pseudomonas aeruginosa和 Bacillus brevis,其次为 Stenotrophomonas mal-tophilia,B.polymyxa,Pseudomonas fluorescens和 Agrobacterium radiobacter及一些还有待进一步鉴定的种 .并对 GC- FAME和 Biolog的鉴定结果进行了比较 :在属的水平上两种方法鉴定一致的拮抗菌株为 14株 ,占 87.5 % ;在种的水平上鉴定一致的拮抗菌株为 7株 ,占 4 2 .75 % .GC- FAME和 Biolog对某些细菌的鉴定提供了快速而可靠的方法 ,但由于目前计算机数据库尚不完备 ,鉴定部分细菌如 B.Gas chromatographic analysis of fatty acid methyl esters (GC FAME),Biolog (computer aided numeric taxonomy method) and other tests were used for identification of 16 antagonistic bacteria against damping off of cucumber seedlings.The results from GC FAME,Biolog and other tests indicated that the dominant species were Pseudomonas aeruginosa and Bacillus brevis ,then Stenotrophomonas maltophilia,B.polymyxa,Pseudomonas fluorescens and Agrobacterium radiobacter and some other isolates showed antagonism which need to be identified.Comparison of the results on identification by both methods GC FAME and Biolog: 14 of 16 antagonistic bacteria showed the same at genus level obtained from two methods,the proportion about 87.5%;then 7 were in species, at 42.75%.GC FAME and Biolog provide rapid and reliable approaches for identification of some bacteria.However,complementary methods are needed for identification of some bacteria such as B.subtilis, due to insufficient database.

关 键 词：GC-FAE BIOLOG 拮抗细菌 鉴定 黄瓜 苗期猝倒病 

分 类 号：S436.421[农业科学—农业昆虫与害虫防治] S432.42[农业科学—植物保护]