在大鼠皮肤切创愈合过程中TGF-β_1基因及蛋白表达变化与损伤时间关系的实验研究  

An experimental study on the gene and protein expression of TGF β_1 and its relationship with the wound age during the healing process of incised wound of rat's skin

作　　者：王慧君[1] 李东[2] 杨木兰[3] 阮海根[4] 黄光照[5] 

机构地区：[1]第一军医大学病理学教研室,广东广州510515 [2]华中科技大学分子生物学研究室,湖北武汉430030 [3]华中科技大学超微病理学研究室,湖北武汉430030 [4]武汉市公安局刑科所,湖北武汉430015 [5]华中科技大学法医学系,湖北武汉430030

出　　处：《中国法医学杂志》2001年第4期193-197,共5页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金资助 ( 39870 76 4);中国博士后科研基金资助 (中博基11)

摘　　要：了解细胞因子在创伤及修复过程中的表达变化与损伤时间的关系。初步调查生前不同造创时间 ( 0 5h～16 8h)皮肤创缘组织在修复过程中TGF β1mRNA和蛋白质表达变化 ,并与死后伤 ( 0 5h～ 6h)上述变化进行比较。结果表明 ,在生前伤 ,TGF β1在上皮细胞的表达于损伤后 0 5h开始增强 ,以 2 4h～ 96h反应最强 ,除上皮细胞内表达外 ,还在肉芽组织的巨噬细胞和成纤维细胞表达。TGF β1的免疫印迹 (WesternBlot)分析表明 ,TGF β1蛋白表达的峰值在 16 8h ;TGF β1斑点杂交和原位杂交结果显示 ,mRNA的表达在伤后 3h开始 ,6h后明显 ,96h达峰值。死后损伤组在 0 5～ 3h内有免疫组化的弱～中度表达 ,但无mRNA的表达 ,3h后则均无任何表达。TGF β1在修复过程中的一些规律性变化及特点反映了与损伤时间的关系 ,可作为精确推断损伤时间的分子生物学标志。In order to explore the relationship between the expression of TGF β 1,its receptor and the wound age during the healing process of rats skin incision wound,a preliminary study were performed on the vital skin wound 0 5～168h after injury by using the immunohistochemical and the molecular biological methods.The results were then compared with those of the postmortem skin injury.The results revealed that the expression of the cytokine TGF β 1 in the epithelial cells were enhanced 0 5h after the antemortem skin incision and the strongest reactions were seen at 24～96h post injury.The expression of TGF β 1 was also found in the macrophages and the fibroblasts in the granulation tissues.Analysis of the immunoblotting(Western blot)results showed that the peak value of the TGF β 1 protein was appeared at 168h after the antemortem skin incision and peaked at 96h.TGF β 1 was detected 0 5～3h after postmorten skin injury by using the immunohistochemical method.No expression of mRNA was found.It is suggested that some regular and characteristic expression of TGF β 1 in the incised skin wounds were related with the wound age.It might be used for timing of skin wound on rat.

关 键 词：法医病理学 TGF-Β1 损伤时间推断 动物实验 细胞因子 

分 类 号：R363[医药卫生—病理学]