大肠癌P21^(WAF1/CIP1)和P53蛋白表达及其与预后的关系  

Expression of P21^(WAF1/CIP1) and P53 and its relation with prognosis in large intestine car-cinoma

作　　者：阎晓初[1] 柳凤轩[1] 贺光友[1] 于冬梅[1] 王宗前[1] 

机构地区：[1]第三军医大学附属西南医院病理学研究所,重庆400038

出　　处：《第三军医大学学报》2001年第8期915-917,共3页Journal of Third Military Medical University

摘　　要：目的 探讨细胞周期调控因子Ｐ２１ＷＡＦ１／ＣＩＰ１和Ｐ５３蛋白表达在大肠癌发生发展中的作用及其临床意义。方法 采用免疫组化染色对１１３ 例大肠癌Ｐ２１和Ｐ５３的蛋白表达进行了检测。结果 高分化腺癌Ｐ２１蛋白阳性表达率显著高于低分化腺癌（Ｐ＜０．０５，大肠癌中Ｐ５３、Ｐ２１ＷＡＦ１／ＣＩＰ１蛋白表达有显著负相关（ｒ＝－０．５８５，Ｐ＜０．０１）。Ｐ５３阳性表达患者的３、５年生存率显著低于阴性表达者（Ｐ＜０．０５），而Ｐ２１阳性表达者高于阴性表达者，且Ｐ２１＋／Ｐ５３－大肠癌死亡率较Ｐ２１－／Ｐ５３＋患者显著降低。结论Ｐ２１蛋白过度表达可能与大肠癌分化有关，ｐ５３基因突变，介导Ｐ２１蛋白产生减少，阻止细胞周期的作用减弱，从而加速大肠癌的发展。Ｐ２１与Ｐ５３蛋白联合检测可能有助于其预后判断。Objective To explore the expression of cell cycle the P21WAF1/CIP1 and P53 in colorectal carcinogenesis, and their clinical sig- nificance. Methods The expressions of P21 and P53 in 113 cases of colorectal carcinomas were detected by immunhistochemical technique. Re- Sults The expression of P21 protein was significantly lower in poorly differentiated adenocarcinomas in well differentiated one (P < 0. 05). Furthermore, P21 and P53 staining were inversely correlated with each other (r = -0.585, P < 0.01). The 3 or 5 year survival rate was signifi- cantly lower in the P53 positive tumor patients than that in P53 negative patients (P < 0.05), the 3 or 5 year survival rate in the patients with P21 strongly positive tumors was higher than that with P21 negative tumors. The mortality in patients with P21 + /P53- tumors was significant lower than that with P21- /P53+ tumors (P < 0.05). Conclusion The overexpression of P21 protein might inhibit the tumor cell proliferation and induce the differentiation of carcinoma cells, and the p53 gene mutation might reduce the expression of P21 protein, and promote the progression of colorectal carcinoma. The combined assessment of P53 and P21 expression might be useful for the prognosis of colorectal carcinoma.

关 键 词：大肠癌 细胞周期 免疫组化 P53蛋白表达 预后 

分 类 号：R735.34[医药卫生—肿瘤]