细胞因子对K562/ADM耐药细胞株耐药性的逆转作用  

The Reversal of multidrug resistance of cytokines and anti-Pgp mAb to the K562/ADM-resistant drug cells

作　　者：肖东杰[1] 孙善会[1] 张红[1] 申红[1] 刘宗印[1] 

机构地区：[1]济南市中心医院生物治疗中心,山东济南250013

出　　处：《细胞与分子免疫学杂志》2001年第6期559-560,共2页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的探讨细胞因子和抗Pgp单抗mAb对K562/ADM耐药细胞株耐药性的逆转作用。方法利用K562细胞的耐阿霉素细胞株K562/ADM采用免疫细胞化学染色法和流式细胞术分别检测mAbMRK-16及rhGM-CSF、rhM-CSF或TNF-α对其Pgp表达的影响。结果将TNF-α100kU/L、rhGM-CSF10μg/L或rhM-CSF10μg/L分别单独与K562/ADM细胞孵育48hPgp表达细胞的阳性率分别为72.19%、80.04%和70.30%与未经任何作用的K562/ADM细胞Pgp表达的阳性率77.02%相比较无明显差异P>0.05。mAbMRK-1610mg/L以及mAbMRK-16分别与TNF-α或rhGM-CSF共同作用于K562/ADM细胞后Pgp表达的阳性率分别为67.49%、67.14%和70.56%与K562/ADM细胞的表达率77.02%相比较Pgp的表达率虽有降低但差别不明显P>0.05只有rhM-CSF与mAbMRK-16共同作用Pgp表达的阳性率为64.29%,可明显抑制K562/ADM细胞上Pgp的表达P<0.05。结论rhM-CSF加mAbMRK-16联合应用,具有逆转K562/ADM细胞MDR的作用。Aim To investigate the reversal of multidrug resistance(MDR) of cytokines and anti-Pgp mAb to the K562/ADM-resistant drug cells. Methods Using multidrug resistant K562/ADM cells(resistant to adriamycin),we detected the influence of rhGM CSF,rhM CSF,TNF α and anti Pgp mAb(mAb MRK16) on Pgp expression by flow cytometry. Results K562/ADM cells were coincubated with TNF α(100 KU/L),rhGM CSF(10 μg/L) or rhM CSF(10 μg/L) for 48 h,the positive rates of Pgp expression were 72.19%,80.04% and 70.30%,respectively, compared with that (77.02%) of the K562/ADM cells, there was no obvious difference(P>0.05). K562/ADM cells were treated with mAb MRK-16(10 mg/L), TNF-α or rhGM-CSF,positive rates of Pgp expression were 67.49%,67.14% and 70.56%,respectively. As compared with positive rate of the Pgp expression on K562/ADM cells, though the positive rate decreased, they hadn’t statistical significantly(P>0.05). However, after K562/ADM cells were treated with rhM-CSF plus mAb MRK-16, Pgp expression decreased significantly(the positive rate was 64.29%),P<0.05.Conclusion Combined the application of rhM-CSF and mAb MRK-16 reverses K562/ADM resistant drug cells to MDR.

关 键 词：K562/ADM细胞 重组M/CSF MRK-16 多药耐药 P-糖蛋白 细胞因子 肿瘤 

分 类 号：R73－36[医药卫生—肿瘤]