人慢性放射性皮肤溃疡端粒酶逆转录酶表达  

Expression of telomerase reverse transcriptase in radiation-induced chronic human skin ulcer

作　　者：赵坡[1] 李志军[1] 吕亚莉[1] 钟梅[1] 谷庆阳[2] 王德文[2] 

机构地区：[1]北京解放军总医院病理科,100853 [2]北京放射医学研究所病理学研究室

出　　处：《中华放射医学与防护杂志》2001年第6期401-403,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目 ( 39470 2 2 9)

摘　　要：目的　调查放射性皮肤溃疡中端粒酶逆转录酶的表达状况与癌变及难于愈合的可能关系。方法　应用兔抗人端粒酶逆转录酶抗体TRT和免疫组化SP方法检测 2 4例福尔马林固定、石蜡包埋的人慢性放射性皮肤溃疡 ,5例正常皮肤 ,2例烧伤皮肤溃疡 ,8例肿瘤标本 ,观察TRT的表达和定位情况。结果　TRT表达阳性率为 5 8 3% (14 2 4) ,其中强阳性占 41 7% (10 2 4) ,弱阳性为16 7% (4 2 4) ,阴性为 41 7% (10 2 4)。阳性部位主要定位于表皮鳞状上皮细胞核及胞质 ;小血管平滑肌、血管内皮细胞、毛细血管内皮细胞和纤维母细胞多为阴性或弱阳性 ;浸润的慢性炎细胞如浆细胞和淋巴细胞呈阳性。TRT强阳性表达多见于病程长、增生旺盛及肥厚的表皮组织 ,而病程短、萎缩的表皮组织多呈阴性或弱阳性。 8例肿瘤组织均为强阳性 ,5例正常皮肤及 2例烧伤皮肤溃疡均为阴性。结论　提示与辐射有关的TRT表达状况可能与放射性皮肤溃疡癌变为鳞状细胞癌的分子机理有关 ;可能与放射性皮肤溃疡小血管硬化、毛细血管及纤维母细胞贫乏即肉芽组织生成不良导致病变难于愈合的机理相关。Objective\ To inverstigate the expression of the catalytic subunit of telomerase,telomerase reverse transcriptase(TRT)and the possible relationship between the TRT and cancer transformation or poor healing in radiation induced chronic ulcer of human skin. Methods\ Rabbit antibody against human TRT and SP immunohistochemical method were used to detect TRT expression in 24 cases of formalin fixed,paraffin embeded human skin chronic ulcer tissues induced by radiation,5 cases of normal skin,2 of burned skin,and 8 of carcinoma. Results\ The positive rate for TRT was 58.3%(14/24)in chronic radiation ulcers,of which the strongly positive rate was 41.7%(10/24) and the weakly positive 16.7%(4/24),0% in normal(0/5)and burned skin(0/2),and 100% in carcinoma(8/8).The strongly positive expression of TRT was observed almost always in the cytoplasm and nucleus of squamous epithelial cells of proliferative epidermis but the negative and partly weakly positive expression in the smooth muscles,endothelia of small blood vessels and capillaries,and fibroblasts.Chronic inflammtory cells,plasmacytes and lymphocytes also showed weakly positive for TRT. Conclusion\ TRT expression could be involved in the malignant transformation of chronic radiation ulcer into squamous carcinoma,and in the poor healing caused by sclerosis of small blood vessels and lack of granulation tissue consisting of capillaries and fibroblasts.\;

关 键 词：皮肤溃疡 端粒酶 放射性 逆转录酶 

分 类 号：R730.2[医药卫生—肿瘤]