室内氡暴露居民肺癌p53和k-ras基因突变  

p53 and k-ras mutations in lung cancer associated with residential radon exposure

作　　者：夏英[1] 杨梅英[2] 吕慧敏[1] 冯治孝 叶常青[2] 王作元[1] 

机构地区：[1]北京卫生部工业卫生实验所,100088 [2]北京放射医学研究所 [3]甘肃庆阳地区医院

出　　处：《中华放射医学与防护杂志》2001年第6期404-406,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目 ( 39970 2 35 )

摘　　要：目的　检测室内高氡暴露地区居民肺癌p5 3和k ras基因突变。方法　采用PCR扩增、PCR SSCP电泳和序列分析方法。结果　 9例标本中有 5例p5 3PCR SSCP电泳呈阳性结果 ,其中 3例经克隆测序发现有碱基置换。结论　室内氡浓度在 2 0 0～ 35 0Bq·m- 3·a- 1 范围内的 7例肺癌病例中有 5例发生p5 3基因突变 ,p5 3基因突变还可能与肺癌患者的年龄及吸烟史有关。Objective\ The aim of this study was to analyze mutation of p53 and k\|ras genes in lung cancer related to residential radon exposure. Methods\ PCR amplification,PCR\|SSCP electrophoresis,and sequence analysis were carried out. Results\ Five of 9 lung cancer cases were found to be positive with p53 PCR\|SSCP electrophoresis techniques,and in 3 among the 5 base substitution was observed. Conclusion\ Five of the seven cases of lung cancer 200 350 Bq·m -3 ·a -1 radon level had p53 gene mutation,which might be related to age and smoking status of the patients.\;

关 键 词：氡 肺癌 P53 PCR-SSCP 序列分析 

分 类 号：R734.2[医药卫生—肿瘤] X591[医药卫生—临床医学]