p53基因功能与肿瘤细胞辐射敏感性及其凋亡效应的研究  

Study on relations between p53 functional status and effects of 60 Co γ-radiation on sensitivity and apoptosis in tumor cells

作　　者：施勤[1] 张学光[1] 许志祥[1] 徐颖[1] 王静[1] 强亦忠[1] 

机构地区：[1]苏州大学免疫研究室,江苏省核医学生物技术重点实验室,江苏215007

出　　处：《中华放射医学与防护杂志》2001年第6期428-430,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：核工业预研基金资助项目 (Y5 5 732 6 2 )

摘　　要：目的　探讨6 0 Coγ射线对不同p5 3基因功能状态的人淋巴性恶性肿瘤细胞株 82 2 6和Raji凋亡相关基因Bcl xL、Bax的作用及其分子机理。方法　分别运用流式细胞仪技术 (FACS)、DNA片段释放法、RT PCR和MTT法 ,分析了 82 2 6、Raji中Bcl xL、Bax转录相对表达与辐照诱导的细胞凋亡率及抑制细胞增殖的关系。结果　 82 2 6和Raji在照射后Bcl xL、Bax转录表达均迅速上升 ,于 4～8h达到高峰 ,而后开始下降 ,于 2 4～ 48h恢复至辐照前水平 ;82 2 6中Bcl xL转录较Raji持续时间为长 ;且Bcl xL Bax的比率影响靶细胞辐照后的细胞凋亡和抑制细胞增殖。结论　肿瘤细胞株p5 3基因状态与辐射敏感性相关 ,两个等位基因突变的细胞株 82 2 6比一个等位基因突变的细胞株Raji更具辐射抗性 ,这种辐射抗性的不同与肿瘤细胞Bcl xL、Bax的表达及二者比率有关。Objective\ To investigate the possible mechanism of the effects of radiation on Bcl\|xL and Bax gene transcription in 8226 and Raji cell lines with different p53 statutes. Methods\ Apoptosis was studied by Annexin\|V\|PI and flow cytometry.Bcl\|xL,Bax gene transcription and the cell apoptosis induced by 60 Co γ\|radiation were analysed by RT\|PCR and DNA released assay,respectively.The proliferation suppression of cells were studied by MTT assay. Results\ The transcriptions of Bcl\|xL and Bax in 8226 and Raji cell lines increased abruptly and reached the their highest points\|4\|8h after γ\|irradiation and then dropped down to the normal levels gradually.The transcriptions of Bcl\|xL Bax and the ratio of Bcl\|xL/Bax were correlated to apoptosis and proliferation suppression of the target cells. Conclusion\ The p53 gene status of the cell lines determines their sensitivity to irradiation.The 8226 cell line,having two p53 allele mutations,is more resistant to ionizing radiation than Raji with one mutation p53 allele.The apoptotic rate is correlated to the transcriptions of Bcl\|xL Bax and the ratio of Bcl\|xL/Bax.\;

关 键 词：P53基因 BCL-XL BAX 辐射敏感性 凋亡 肿瘤细胞 

分 类 号：R730.5[医药卫生—肿瘤]