维拉帕米抑制Lewis肺癌自发性转移作用的实验研究  

An experimental study on the inhibition of spontaneous tumor metastasis of Lewis lung carcinoma by the calcium antagonist verapamil

作　　者：赵环宇[1] 张昌义[2] 

机构地区：[1]南京医科大学第二附属医院灵顿中子刀研究治疗中心 [2]青岛大学医学院附属医院肿瘤科,青岛266003

出　　处：《临床肿瘤学杂志》2002年第1期5-7,共3页Chinese Clinical Oncology

摘　　要：目的 :观察不同剂量的维拉帕米对Lewis肺癌自发性肺转移的抑制作用。方法 :将Lewis肺癌细胞种植于C57BL/ 6J小鼠右腹股沟皮下 (2× 10 6/鼠 ) ,将 4 0只小鼠随机分成对照组和 3个治疗组 ,从种植第 5天起腹腔内分别注射生理盐水、2 0mg/kg、30mg/kg和 4 0mg/kg维拉帕米 ,每日 1次 ,共 11天 ,第 2 2天处死并解剖小鼠 ,观察对照组和治疗组的肺转移情况 ,体外进行血小板聚集抑制试验。结果 :治疗组肺转移率明显低于对照组 ,随着药物剂量的增加 ,肺转移率逐渐降低 (P <0 0 1) ;维拉帕米可抑制Lewis肺癌细胞诱致的人血小板聚集 ,抑制率随着药物浓度的增加而增加 (P <0 0 1)。结论 :维拉帕米可剂量依赖性地抑制Lewis肺癌自发性肺转移 。Objective:To observe the inhibition of spontaneous lung metastasis in Lewis lung carcinoma by different doses of verapamil.Methods:Lewis lung carcinoma cells(2×10 6/mouse)were inoculated subcutaneously into the right inguen of 40 C 57 BL/6J mice,which were randomized into 4 groups.From the 5th day,the treated groups were given 20mg/kg?30mg/kg and 40mg/kg of verapamil IP respectively once a day for 11 days,and the control group was given physiological saline only.On the 22nd day,the mice were killed and dissected,the lung metastasis of the 4 groups were detected,the inhibition of tumor cells induced platelet aggregation by verapamil was examined in vitro.Results:The rates of lung metastasis of the treated groups were much less than that of the control,along with the increasedose of Verapamil,was the rates of lung metastasis decreased( P <0.01).Verapamil inhibited the platelet aggregation induced by Lewis lung carcinoma cells,the inhibition was dependent on the concentration of the drug.Conclusion:Verapamil can dose-dependently inhibits the spontaneous lung metastasis of Lewis lung carcinoma,the function of its antimetastasis may be related to the inhibition of tumor cells induced platelet aggregation.

关 键 词：钙拮抗剂 维拉帕米 肺癌 肿瘤转移 控制 

分 类 号：R734.201[医药卫生—肿瘤]