肾细胞癌bcl-2及ki-67的免疫组化研究  

Immunohistochemical Study of bcl-2 and ki-67 in Human Renal Cell Carcinoma

作　　者：邹高德[1] 葛根[1] 孙锡林[1] 

机构地区：[1]江西医学院第二附属医院泌尿外科,江西南昌330006

出　　处：《江西医学院学报》2002年第1期33-35,共3页Acta Academiae Medicinae Jiangxi

摘　　要：目的 :探讨bcl 2和ki 6 7基因产物与肾癌生物学行为及预后的关系。方法 :采用免疫组化LSAB法对 6 0例肾细胞癌和 5例正常肾组织bcl 2及ki 6 7表达产物进行检测。结果 :6 6 .7%肾癌bcl 2显示阳性 ,正常肾组织均为阴性。bcl 2表达与肾癌的分期、分级有关 ,且随着肾癌分期、分级的上升而降低 (P <0 .0 1) ;bcl 2表达与肾癌组织学类型无关 ;bcl 2阳性表达者预后较好。Ki 6 7在正常肾组织中低表达。在全部肾癌中ki 6 7均表达 ,程度不同 ,与分期、分级密切相关 (P <0 .0 0 5 ) ,与组织学类型无关 ;ki 6 7高表达者预后差。结论 :bcl 2及ki 6 7均可作为肾癌恶性程度及预后的估价性指标 。Objective: To study the correlation of bcl 2 and ki 67 with biological behaviors and prognosis of renal cell carcinomas (RCC).Methods:The expressions of bcl 2 and ki 67 in 60 RCC and 5 normal renal tissue specimens were detected by immunohistochemical method,LSAB.Results:Positive staining of bcl-2 protein was found in 66.7% of RCC (40/60), while none in normal renal tissues. Expression of bcl 2 was closely related to the clinical stage and cell grading. The expression of bcl 2 gene was decreased with the advances of stage and grading( P <0.01). No correlation was found between bcl 2 expression and histology type.The cases with negative bcl 2 expression had a poor prognosis. Although expression of ki 67 was found in all RCC, there was a faint stain in normal renal tissue.The expression of ki 67 was closely related to the clinical stage and grading ( P <0.005). No correlation was found between ki 67 and histology type. The cases with a higher expression had poor prognosis.Conclusion:bcl 2 and ki 67 might be applicable as tumor markers for the evaluation of malignant potential and prognosis of RCC.

关 键 词：肾癌 免疫组织化学 BCL-2 KI-67 肾细胞癌 预后 恶性程度 基因表达 RCC 

分 类 号：R737.11[医药卫生—肿瘤]