低温对脑缺血再灌注期间微管相关蛋白-2免疫活性的影响  

Effect of hypothermia on the immunoreactivity of microtubule associated protein 2 during cerebral ischemia/reperfusion

作　　者：顾卫东[1] 陈群[2] 曾因明[2] 丁浩中[1] 

机构地区：[1]无锡市第四人民医院麻醉科,江苏无锡214062 [2]徐州医学院江苏省麻醉学重点实验室,江苏徐州221002

出　　处：《徐州医学院学报》2002年第1期14-17,共4页Acta Academiae Medicinae Xuzhou

基　　金：江苏省青年科技基金资助课题 (BQ980 0 9)

摘　　要：目的　观察低温对脑缺血再灌注期间海马CA1区微管相关蛋白 - 2免疫活性的影响。方法　沙土鼠前脑缺血再灌注模型。动物随机分为假手术组、常温再灌组和低温再灌组 ,后两组又各分为再灌注 6h、4 8h、96h3个亚组 ,每组 10只。采用免疫组织化学方法结合计算机图像分析测定微管相关蛋白 - 2活性 ,观察再灌注 4 8h、96h海马CA1区细胞膜、核膜完整 ,核仁清楚的锥体神经元数目。结果　常温再灌注 6h、4 8h、96h海马CA1区微管相关蛋白 - 2免疫活性分别为假手术组的 81%、6 9%和 5 1% (P <0 .0 1)。低温再灌注 6h、4 8h、96h海马CA1区微管相关蛋白 - 2免疫活性分别为假手术组的 93%、86 %和 71% ,均明显高于常温再灌组 (P <0 .0 5或P<0 .0 1)。再灌注 96h时 ,常温再灌组海马CA1区细胞膜、核膜完整 ,核仁清楚的锥体细胞数目仅为假手术组的5 % (P <0 .0 1) ,低温再灌组为假手术组的 4 7% ,明显多于常温再灌组 (P <0 .0 1)。结论　低温减轻脑缺血后延迟性神经元死亡的作用可能与其抑制微管相关蛋白 -Objective To investigate the effect of hypothermia on the immunoreactivity of microtubule associated protein 2 (MAP2) during cerebral ischemia/reperfusion. Methods The cerebral ischemia models of gerbils were achieved by occluding bilateral carotid arteries, and the insult time was 10 min. The gerbils were randomly divided into sham-operation group, normothermia reperfusion group and hypothermia reperfusion group. The normothermia and hypothermia reperfusion groups were further divided into subgroups according to the reperfusion time (6 h, 48 h and 96 h). The immunoreactivity of MAP2 was determined by using immunohistochemical staining and computer image analysis system. The survival neurons in hippocampal CA1 were counted 48 h and 96 h after reperfusion. Results The immunoreactivity of MAP2 in hippocampal CA1 6 h, 48 h and 96 h after reperfusion in normothermia reperfusion group were decreased to 81%, 69% and 51% that in sham-operation group (P<0.01). The immunoreactivities of MAP2 in hippocampal CA1 in hypothermia reperfusion group were 93%, 86% and 71% that in sham-operation group, much higher than that in normothermia reperfusion group (P<0.05 or P<0.01). The number of survival neurons in hippocampal CA1 96 h after reperfusion in normothermia reperfusion group was only 5% that in sham-operation group (P<0.01), while the number of survival neurons 96 h after reperfusion in hypothermia reperfusion group was 47%, much more than that in normothermia reperfusion group (P<0.01). Conclusion Hypothemia could attenuate neuronal damage through inhibition of MAP2 degradation during cerebral ischemia/reperfusion.

关 键 词：延迟性神经元死亡 低温 微管相关蛋白-2 脑缺血 

分 类 号：R743.31[医药卫生—神经病学与精神病学]