西藏部分地区豆科植物根瘤菌资源的初步调查  

Investigation of rhizobial resources in the Tibet Autonomous Region

作　　者：王素英[1] 李润花[2] 刘新成[3] 李新锁 

机构地区：[1]天津商学院食品与生物工程系,天津300400 [2]山西教育学院生物系,山西太原030000 [3]天津师范大学化学与生命科学学院,天津300074

出　　处：《西北农林科技大学学报（自然科学版）》2002年第1期33-37,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金资助项目 ( 39970 0 17)

摘　　要：对采自西藏拉萨、林芝和日喀则地区的 10属 18种豆科植物的 2 82份根瘤样品进行根瘤菌的分离纯化、菌株的回接和固氮酶活性的测定 ,结果表明 ,这些地区的豆科植物均可自然结瘤 ,其形态比较单一 ,主要为较规则的球形、柱状和掌状 ,颜色为褐红色、红色、粉红色和白色 ,球形根瘤的大小为 Φ0 .1～ 6 .0 m m,柱状根瘤的大小为 1～ 10 mm,92 .5 %的自然根瘤可测到固氮酶活性 ,其活性值为 0 .0 93～ 12 .40 3nmol/ (mg· h) ,可以看出 ,豆科植物根瘤的大小、颜色和固氮酶的活性与采集地点的生态环境相关。specimens of root nodule,Which are dispersed over 10 genera 18 species of legumes in the Lasha,Linzhi and Rikaze prefecture of Tibet Autonomous Region,were analysed by isolation,purification,renodulation of rhizobial strains and determination of nitrogenase activity.The results show that all leguminous plants could nodulate under the natural environment,the shape of nodules is mainly spherical,cylindrical and palmate,and the color is brown,red and pink,the diameter of spheriod nodule is 0.1- 6.0 mm and the length of cylinder and palm nodule is 1-10 mm.92.5% natural nodules have activity of nitrogen fixation,and the value of nitrogenase activity is 0.093-12.403 nmol/(mg·h).It is obvious that the shape,color,size and nitrogenase activity of nodule are related to ecological environment of sampling sites.

关 键 词：西藏 豆科植物 根瘤菌 固氮状况 分离 纯化 固氮酶活性 

分 类 号：Q939.114[生物学—微生物学] Q948.122.3